Selective-plane-activation Structured Illumination Microscopy

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2024 Apr 5

PMID 38580844

Authors

Kenta Temma

Ryosuke Oketani

Toshiki Kubo

Kazuki Bando

Shunsuke Maeda

Kazunori Sugiura

Tomoki Matsuda

Rainer Heintzmann

Tatsuya Kaminishi

Koki Fukuda

Maho Hamasaki

Takeharu Nagai

Katsumasa Fujita

Affiliations

Soon will be listed here.

Abstract

The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids.

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