Base Composition-independent Hybridization in Tetramethylammonium Chloride: a Method for Oligonucleotide Screening of Highly Complex Gene Libraries

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Mar 1

PMID 3856838

Citations 288

Authors

W I Wood

J Gitschier

L A Lasky

R M Lawn

Affiliations

Soon will be listed here.

Abstract

An oligonucleotide hybridization procedure has been developed that eliminates the preferential melting of A X T versus G X C base pairs, allowing the stringency of the hybridization to be controlled as a function of probe length only. This technique, which uses tetramethylammonium chloride, is especially helpful whenever a highly complex library is screened with a pool of oligonucleotide probes, which usually vary widely in base composition. The procedure can also be applied advantageously whenever an exact match to an oligonucleotide probe is desired, such as in screening for clones having as little as a single-base alteration generated by in vitro mutagenesis.

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