Novel Erectile Analyses Revealed Augmentable Penile Lyve-1, the Lymphatic Marker, Expression

Overview

Journal Reprod Med Biol

Specialty Biology

Date 2024 Apr 3

PMID 38566911

Authors

Kota Fujimoto

Daiki Hashimoto

Sang Woon Kim

Yong Seung Lee

Takuya Suzuki

Masanori Nakata

Shinji Kumegawa

Shinichi Asamura

Gen Yamada

Affiliations

Soon will be listed here.

Abstract

Purpose: The pathophysiology of penis extends to erectile dysfunction (ED) to conditions including sexually transmitted diseases (STDs) and cancer. To date, there has been little research evaluating vascular drainage from the penis. We aimed to evaluate penile blood flow in vivo and analyze its possible relationship with the lymphatic maker.

Materials And Methods: We established an in vivo system designed to assess the dynamic blood outflow from the corpus cavernosum (CC) by dye injection. To analyze lymphatic characteristics in the CC, the expression of Lyve-1, the key lymphatic endothelium marker, was examined by the in vitro system and lipopolysaccharide (LPS) injection to mimic the inflammatory conditions.

Results: A novel cavernography methods enable high-resolution morphological and functional blood drainage analysis. The expression of Lyve-1 was detected along the sinusoids. Furthermore, its prominent expression was also observed after penile LPS injection and in the erectile condition.

Conclusions: The current in vivo system will potentially contribute to the assessment of penile pathology from a novel viewpoint. In addition, current analyses revealed inducible Lyve-1 expression for LPS injection and the erection state, which requires further analyses on penile lymphatic system.

Citing Articles

Developmental and functional roles of androgen and interactive signals for external genitalia and erectile tissues.

Hashimoto D, Fujimoto K, Nakata M, Suzuki T, Kumegawa S, Ueda Y Reprod Med Biol. 2024; 23(1):e12611.

PMID: 39372370 PMC: 11456227. DOI: 10.1002/rmb2.12611.

Novel erectile analyses revealed augmentable penile Lyve-1, the lymphatic marker, expression.

Fujimoto K, Hashimoto D, Kim S, Lee Y, Suzuki T, Nakata M Reprod Med Biol. 2024; 23(1):e12570.

PMID: 38566911 PMC: 10985380. DOI: 10.1002/rmb2.12570.

References

Feldman H, Goldstein I, Hatzichristou D, Krane R, McKinlay J . Impotence and its medical and psychosocial correlates: results of the Massachusetts Male Aging Study. J Urol. 1994; 151(1):54-61. DOI: 10.1016/s0022-5347(17)34871-1. View

Jena A, Goldman D, Kamdar A, Lakdawalla D, Lu Y . Sexually transmitted diseases among users of erectile dysfunction drugs: analysis of claims data. Ann Intern Med. 2010; 153(1):1-7. PMC: 3673772. DOI: 10.7326/0003-4819-153-1-201007060-00003. View

Bayraktar N . Non-invasive alternative for phosphodiesterase inhibitor-refractory erectile dysfunction: Real-life experience with low-intensity extracorporeal shockwave therapy. Medicine (Baltimore). 2023; 102(45):e35939. PMC: 10637505. DOI: 10.1097/MD.0000000000035939. View

Suh C, Baheti A, Tirumani S, Rosenthal M, Kim K, Ramaiya N . Multimodality imaging of penile cancer: what radiologists need to know. Abdom Imaging. 2014; 40(2):424-35. DOI: 10.1007/s00261-014-0218-6. View

Soleiman A, Kowalski H, Horvat R, Amann G, Kriehuber E, Diem K . Angiosarcomas express mixed endothelial phenotypes of blood and lymphatic capillaries: podoplanin as a specific marker for lymphatic endothelium. Am J Pathol. 1999; 154(2):385-94. PMC: 1849992. DOI: 10.1016/S0002-9440(10)65285-6. View

Muneer A, Kalsi J, Nazareth I, Arya M . Erectile dysfunction. BMJ. 2014; 348:g129. DOI: 10.1136/bmj.g129. View

Yin G, Das N, Choi M, Song K, Kwon M, Ock J . The pericyte as a cellular regulator of penile erection and a novel therapeutic target for erectile dysfunction. Sci Rep. 2015; 5:10891. PMC: 4456662. DOI: 10.1038/srep10891. View

Wigle J, Oliver G . Prox1 function is required for the development of the murine lymphatic system. Cell. 1999; 98(6):769-78. DOI: 10.1016/s0092-8674(00)81511-1. View

Gonzalez-Loyola A, Petrova T . Development and aging of the lymphatic vascular system. Adv Drug Deliv Rev. 2020; 169:63-78. DOI: 10.1016/j.addr.2020.12.005. View

10.

Hashimoto D, Hirashima T, Yamamura H, Kataoka T, Fujimoto K, Hyuga T . Dynamic erectile responses of a novel penile organ model utilizing TPEM†. Biol Reprod. 2021; 104(4):875-886. DOI: 10.1093/biolre/ioab011. View

11.

Alitalo K . The lymphatic vasculature in disease. Nat Med. 2011; 17(11):1371-80. DOI: 10.1038/nm.2545. View

12.

Torbrand C, Wigertz A, Drevin L, Folkvaljon Y, Lambe M, Hakansson U . Socioeconomic factors and penile cancer risk and mortality; a population-based study. BJU Int. 2016; 119(2):254-260. DOI: 10.1111/bju.13534. View

13.

Bivalacqua T, Ross A, Strong T, Gebska M, Musicki B, Champion H . Attenuated RhoA/Rho-kinase signaling in penis of transgenic sickle cell mice. Urology. 2010; 76(2):510.e7-12. PMC: 3762569. DOI: 10.1016/j.urology.2010.02.050. View

14.

Lagoda G, Sezen S, Cabrini M, Musicki B, Burnett A . Molecular analysis of erection regulatory factors in sickle cell disease associated priapism in the human penis. J Urol. 2012; 189(2):762-8. PMC: 4478587. DOI: 10.1016/j.juro.2012.08.198. View

15.

Pepper M, Skobe M . Lymphatic endothelium: morphological, molecular and functional properties. J Cell Biol. 2003; 163(2):209-13. PMC: 2173536. DOI: 10.1083/jcb.200308082. View

16.

Nishioka Y, Tamai K, Onda M, Hiromori Y, Kimura T, Hu J . Potential Interference of Oil Vehicles on Genital Tubercle Development during the Fetal Period in ICR Mice. Biol Pharm Bull. 2018; 41(2):266-271. DOI: 10.1248/bpb.b17-00830. View

17.

Huang T, Wang G, Hu Y, Shi H, Wang K, Yin L . Structural and functional abnormalities of penile cavernous endothelial cells result in erectile dysfunction at experimental autoimmune prostatitis rat. J Inflamm (Lond). 2019; 16:20. PMC: 6659287. DOI: 10.1186/s12950-019-0224-0. View

18.

Kumegawa S, Yamada G, Hashimoto D, Hirashima T, Kajimoto M, Isono K . Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model. Biol Proced Online. 2021; 23(1):21. PMC: 8582144. DOI: 10.1186/s12575-021-00159-3. View

19.

Fujimoto K, Hashimoto D, Kashimada K, Kumegawa S, Ueda Y, Hyuga T . A visualization system for erectile vascular dynamics. Front Cell Dev Biol. 2022; 10:1000342. PMC: 9615422. DOI: 10.3389/fcell.2022.1000342. View

20.

Yafi F, Jenkins L, Albersen M, Corona G, Isidori A, Goldfarb S . Erectile dysfunction. Nat Rev Dis Primers. 2016; 2:16003. PMC: 5027992. DOI: 10.1038/nrdp.2016.3. View