The Complete Sequence of the Chicken Alpha-cardiac Actin Gene: a Highly Conserved Vertebrate Gene

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1985 Feb 25

PMID 3855241

Citations 33

Authors

K S Chang

K N Rothblum

R J Schwartz

Affiliations

Soon will be listed here.

Abstract

We sequenced the entire chicken alpha-cardiac actin gene. A single intron was positioned 20 bp upstream from the initiation ATG codon in the 5' non-coding region while the coding region was interrupted by 5 introns at amino acid positions 41/42, 150, 204, 267, and 327/328. Sequencing allowed the first comparison of the alpha-cardiac and alpha-skeletal actin transcriptional promoters. These highly G+C rich promoters share two regions of homology which are found at position -134 (10 bp) and -296 (12 bp) in the alpha-cardiac actin promoter. A smaller 9 bp motif (CCGCGCCGG) homologous to the -134 sequence was detected before, between and after the TATA and CAAT boxes of the alpha-cardiac actin gene. The polyadenylation signal (AATAAA) was located 156 bp downstream from the translation termination codon. The complete length of the alpha-cardiac actin mRNA excluding the poly A tail is 1370 nucleotides. The 3' noncoding transcribed portion of the chicken alpha-cardiac actin gene was found to be extraordinarily conserved when compared to the human and rat alpha-cardiac actin mRNA sequences.

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