Molecular Detection of Extended-spectrum β-lactamase-producing from Bat Caves on Lombok Island

Overview

Journal Open Vet J

Specialty Veterinary Medicine

Date 2024 Mar 29

PMID 38549568

Authors

Yolla Rona Mustika

Kurnia Nisa Kinasih

Mustofa Helmi Effendi

Yulianna Puspitasari

Shendy Canadya Kurniawan

Aswin Rafif Khairullah

Muhammad Esa Erlang Samodra

Abdullah Hasib

Alfiana Laili Dwi Agustin

Ikechukwu Benjamin Moses

Otto Sahat Martua Silaen

Affiliations

Soon will be listed here.

Abstract

Background: The discovery of antibiotic-resistant Enterobacteriaceae bacteria in wild animals is an indication of their potential for wildlife as a reservoir. Bats are natural reservoir hosts and a source of infection for several microorganisms and have the potential to become vectors for the spread of zoonotic diseases.

Aim: A study was conducted based on these characteristics to identify and detect the TEM gene in isolated from bat excrements in Tanjung Ringgit Cave, East Lombok.

Methods: Bat fecal samples were firstly inoculated onto eosin methylene blue agar media. Recovered bacterial isolates were further characterized using standard microbiological techniques. Antimicrobial susceptibility testing was done using the Kirby-Bauer disc diffusion method. TEM gene detection was carried out using polymerase chain reaction (PCR).

Results: Out of the 150 bat fecal samples obtained from Tanjung Ringgit cave, Lombok Island, Indonesia, 56 (37%) were positive for . Eight (8) out of the 56 isolates that underwent antimicrobial susceptibility testing using the disc diffusion method were confirmed to be multidrug-resistant as they exhibited resistance to at least three different classes of antibiotics. Out of the eight (8) multidrug resistance isolates recovered from fecal samples of bats, 2 (two) harbored the TEM gene.

Conclusion: The discovery of the TEM gene in bat fecal samples indicates the potential for wild animals, especially bats, to spread ESBL resistance genes to the environment and to humans.

Citing Articles

Phylogenetic analysis TEM gene of isolated from cave bats in West Nusa Tenggara Province, Indonesia.

Agustin A, Effendi M, Tyasningsih W, Plumeriastuti H, Khairullah A, Ekawasti F Open Vet J. 2025; 14(12):3460-3473.

PMID: 39927370 PMC: 11799622. DOI: 10.5455/OVJ.2024.v14.i12.30.

Extended-spectrum β-lactamase-producing from poultry: A review.

Widodo A, Khairullah A, Effendi M, Moses I, Agustin A Vet World. 2024; 17(9):2017-2027.

PMID: 39507773 PMC: 11536724. DOI: 10.14202/vetworld.2024.2017-2027.

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