RNA-Seq Analysis Reveals the Molecular Mechanisms Regulating the Development of Different Adipose Tissues in Broiler Chicks

Overview

Journal Animals (Basel)

Date 2024 Mar 28

PMID 38539997

Authors

Shuo Wei

Xincheng Kang

Felix Kwame Amevor

Xiaxia Du

Youhao Wu

Zhengyu Xu

Xueqing Cao

Gang Shu

Xiaoling Zhao

Affiliations

Soon will be listed here.

Abstract

In an effort to enhance growth rates, chicken breeders have undertaken intensive genetic selection. In the selection process, the primary aim is to accelerate growth, inadvertently leading to new chicken breeds having an increased capacity for rapid adipose tissue accumulation. However, little is known about the relationship between changes in gene expression and adipose tissue accumulation and deposition in chickens. Therefore, in this study, RNA-seq analysis was utilized, and transcriptome data were obtained from the abdominal fat, thoracic subcutaneous fat, and clavicular fat on day 1 (d1), day 4, day 7, day 11, and day 15 to reveal the molecular mechanisms regulating the development and deposition of different adipose tissues in broiler chicks. The results showed that the key period for adipocyte differentiation and proliferation was between d4 and d7 (abdominal fat development) and between d1 and d4 (chest subcutaneous fat and clavicular fat). In addition, candidate genes such as , , , , and related to adipose tissue growth and development were identified. Further, genes (, , , , , and ) associated with the distribution of adipose tissue were identified, and genes (, , and ) related to adipose tissue growth were also identified. Taken together, the results from this study provide the basis for future studies on the mechanisms regulating adipose tissue development in chickens. Further, the candidate genes identified could be used in the selection process.

Citing Articles

Population Structure and Selection Signatures in Chinese Indigenous Zhaotong Pigs Revealed by Whole-Genome Resequencing.

Zhu Y, Wang X, Yang Y, Wang L, Xu C, Xu W Animals (Basel). 2024; 14(21).

PMID: 39518852 PMC: 11544797. DOI: 10.3390/ani14213129.

References

Xu T, Gu L, Schachtschneider K, Liu X, Huang W, Xie M . Identification of differentially expressed genes in breast muscle and skin fat of postnatal Pekin duck. PLoS One. 2014; 9(9):e107574. PMC: 4180276. DOI: 10.1371/journal.pone.0107574. View

Ojima K, Muroya S, Wada H, Ogawa K, Oe M, Takimoto K . Immature adipocyte-derived exosomes inhibit expression of muscle differentiation markers. FEBS Open Bio. 2021; 11(3):768-781. PMC: 7931241. DOI: 10.1002/2211-5463.13100. View

Ono M, Aratani Y, Kitagawa I, Kitagawa Y . Ascorbic acid phosphate stimulates type IV collagen synthesis and accelerates adipose conversion of 3T3-L1 cells. Exp Cell Res. 1990; 187(2):309-14. DOI: 10.1016/0014-4827(90)90096-s. View

Fouad A, El-Senousey H . Nutritional factors affecting abdominal fat deposition in poultry: a review. Asian-Australas J Anim Sci. 2014; 27(7):1057-68. PMC: 4093572. DOI: 10.5713/ajas.2013.13702. View

Deng J, Guo Y, Yuan F, Chen S, Yin H, Jiang X . Autophagy inhibition prevents glucocorticoid-increased adiposity via suppressing BAT whitening. Autophagy. 2019; 16(3):451-465. PMC: 6999619. DOI: 10.1080/15548627.2019.1628537. View

Zhang X, Wu M, Wang S, Zhang H, Du Z, Li Y . Genetic selection on abdominal fat content alters the reproductive performance of broilers. Animal. 2017; 12(6):1232-1241. DOI: 10.1017/S1751731117002658. View

Mortazavi A, Williams B, McCue K, Schaeffer L, Wold B . Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 2008; 5(7):621-8. DOI: 10.1038/nmeth.1226. View

Zhao L, Liu S, Zhang Z, Zhang J, Jin X, Zhang J . Low and high concentrations of butyrate regulate fat accumulation in chicken adipocytes via different mechanisms. Adipocyte. 2020; 9(1):120-131. PMC: 7153540. DOI: 10.1080/21623945.2020.1738791. View

San J, Du Y, Wu G, Xu R, Yang J, Hu J . Transcriptome analysis identifies signaling pathways related to meat quality in broiler chickens - the extracellular matrix (ECM) receptor interaction signaling pathway. Poult Sci. 2021; 100(6):101135. PMC: 8105667. DOI: 10.1016/j.psj.2021.101135. View

10.

Xu S, Chang Y, Wu G, Zhang W, Man C . Potential role of miR-155-5p in fat deposition and skeletal muscle development of chicken. Biosci Rep. 2020; 40(6). PMC: 7269915. DOI: 10.1042/BSR20193796. View

11.

Qiu F, Xie L, Ma J, Luo W, Zhang L, Chao Z . Lower Expression of Enhances Intramuscular Fat Deposition in Chicken via Down-Regulated Fatty Acid Oxidation Mediated by . Front Physiol. 2017; 8:449. PMC: 5489693. DOI: 10.3389/fphys.2017.00449. View

12.

Xiao Y, Wang G, Gerrard M, Wieland S, Davis M, Cline M . Changes in adipose tissue physiology during the first two weeks posthatch in chicks from lines selected for low or high body weight. Am J Physiol Regul Integr Comp Physiol. 2019; 316(6):R802-R818. PMC: 6620650. DOI: 10.1152/ajpregu.00017.2019. View

13.

Kim J, Park K, Lee E, Jang W, Seo J, Shin S . Suppression of PPARγ through MKRN1-mediated ubiquitination and degradation prevents adipocyte differentiation. Cell Death Differ. 2013; 21(4):594-603. PMC: 3950322. DOI: 10.1038/cdd.2013.181. View

14.

Wan X, Yang Z, Ji H, Li N, Yang Z, Xu L . Effects of lycopene on abdominal fat deposition, serum lipids levels and hepatic lipid metabolism-related enzymes in broiler chickens. Anim Biosci. 2020; 34(3):385-392. PMC: 7961199. DOI: 10.5713/ajas.20.0432. View

15.

Li H, Sun J, Li B, Jiang A, Tao J, Ning C . AMPK-PPARγ-Cidec Axis Drives the Fasting-Induced Lipid Droplet Aggregation in the Liver of Obese Mice. Front Nutr. 2022; 9:917801. PMC: 9289538. DOI: 10.3389/fnut.2022.917801. View

16.

Liu X, Wang C, Wang Y, Wang C, Sun X, Zhu Y . Age-associated changes in the growth development of abdominal fat and their correlations with cecal gut microbiota in broiler chickens. Poult Sci. 2023; 102(9):102900. PMC: 10466292. DOI: 10.1016/j.psj.2023.102900. View

17.

Ceylan-Isik A, Kandadi M, Xu X, Hua Y, Chicco A, Ren J . Apelin administration ameliorates high fat diet-induced cardiac hypertrophy and contractile dysfunction. J Mol Cell Cardiol. 2013; 63:4-13. DOI: 10.1016/j.yjmcc.2013.07.002. View

18.

Zhuo Z, Lamont S, Lee W, Abasht B . RNA-Seq Analysis of Abdominal Fat Reveals Differences between Modern Commercial Broiler Chickens with High and Low Feed Efficiencies. PLoS One. 2015; 10(8):e0135810. PMC: 4546421. DOI: 10.1371/journal.pone.0135810. View

19.

Farmer S . Transcriptional control of adipocyte formation. Cell Metab. 2006; 4(4):263-73. PMC: 1958996. DOI: 10.1016/j.cmet.2006.07.001. View

20.

Rao X, Huang X, Zhou Z, Lin X . An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinforma Biomath. 2015; 3(3):71-85. PMC: 4280562. View