Knockdown of the Expression of Two Trehalase Genes with RNAi Disrupts the Trehalose and Chitin Metabolism Pathways in the Oriental Armyworm,

Overview

Journal Insects

Specialty Biology

Date 2024 Mar 27

PMID 38535338

Authors

Hongjia Yang

Yixiao Wang

Weijia Zhang

Xinxin Zhang

Sibo Wang

Mengyao Cui

Xiaohui Zhao

Dong Fan

Changchun Dai

Affiliations

Soon will be listed here.

Abstract

Trehalose is an important carbohydrate substance in insect hemolymph. Chitin is the main component of cuticle and peritrophic matrix in insects. Trehalase (Tre) catalyzes the decomposition of trehalose. Few studies of trehalase in lepidopteran insects have been conducted. Here, the functions of soluble Tre (Tre1) and membrane-bound Tre (Tre2) in the growth and development of were investigated. We cloned and identified and cDNA sequences in . Analysis expression revealed that and were highly expressed in midgut and integument, respectively. The expression of and was highest in the pupal stage. We used RNA interference (RNAi) to inhibit expression in larvae. Injection of ds or ds resulted in abnormal phenotypes and impeded normal molting. Silencing of and resulted in significant changes in the expression of genes in the trehalose and chitin metabolism pathways, significantly increased the trehalose and glycogen content, and significantly decreased MsTre1 and MsTre2 activity, the glucose content, and the chitin content in midgut and integument. Silencing of slowed larval molting, and the new cuticle was significantly thinner. These results indicate that RNAi of may be useful for control strategies against .

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