Long Non-coding RNA-NONMMMUT004552.2 Regulates the Unloading-induced Bone Loss Through the MiRNA-15b-5p/Syne1 in Mice

Overview

Journal NPJ Microgravity

Publisher Springer Nature

Date 2024 Mar 24

PMID 38521778

Authors

Zheng Zhang

Yu Jing

Ang Zhang

Jishan Liu

Heming Yang

Xiaotong Lou

Liyan Xu

Min Liu

Yikun Zhang

Jianwen Gu

Affiliations

Soon will be listed here.

Abstract

Exercise-induced mechanical loading can increase bone strength whilst mechanical unloading enhances bone-loss. Here, we investigated the role of lncRNA NONMMUT004552.2 in unloading-induced bone-loss. Knockout of lncRNA NONMMUT004552.2 in hindlimb-unloaded mice caused an increase in the bone formation and osteoblast activity. The silencing of lncRNA NONMMUT004552.2 also decreased the osteoblast apoptosis and expression of Bax and cleaved caspase-3, increased Bcl-2 protein expression in MC3T3-E1 cells. Mechanistic investigations demonstrated that NONMMUT004552.2 functions as a competing endogenous RNA (ceRNA) to facilitate the protein expression of spectrin repeat containing, nuclear envelope 1 (Syne1) by competitively binding miR-15b-5p and subsequently inhibits the osteoblast differentiation and bone formation in the microgravity unloading environment. These data highlight the importance of the lncRNA NONMMUT004552.2/miR-15b-5p/Syne1 axis for the treatment of osteoporosis.

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