Establishment and Application of a Reverse Dot Blot Assay for 13 Mutations of Hearing-loss Genes in Primary Hospitals in China

Overview

Journal Asian Biomed (Res Rev News)

Date 2024 Mar 22

PMID 38515630

Authors

Qing-Qing Jiang

Juan-Juan Zhu

Shu-Ling Fan

Ya-Ping Hou

Xie-Ying Hu

Jie Shi

Lei Wu

Ying Luo

Affiliations

Soon will be listed here.

Abstract

Background: Hearing loss is a common sensorineural dysfunction with a high incidence in China. Although genetic factors are important causes of hearing loss, hearing-related gene detection has not been widely adopted in China.

Objective: Establishing a rapid and efficient method to simultaneously detect hotspot hearing loss gene mutations.

Methods: A reverse dot blot assay combined with a flow-through hybridization technique was developed for the simultaneous detection of 13 hotspot mutations of 4 hearing loss-related genes including and the mitochondrial gene . This method involved PCR amplification systems and a hybridization platform.

Results: The technique can detect 13 hotspot mutations of 4 hearing loss-related genes. And a total of 213 blood samples were used to evaluate the availability of this method.

Discussion: Our reverse dot blot assay was a simple, rapid, accurate, and cost-effective method to identify hotspot mutations of 4 hearing loss-related genes in a Chinese population.

Citing Articles

Early detection and management of hearing loss to improve the quality of life.

Asian Biomed (Res Rev News). 2024; 18(1):1.

PMID: 38515635 PMC: 10953841. DOI: 10.2478/abm-2024-0001.

Establishment and application of a reverse dot blot assay for 13 mutations of hearing-loss genes in primary hospitals in China.

Jiang Q, Zhu J, Fan S, Hou Y, Hu X, Shi J Asian Biomed (Res Rev News). 2024; 18(1):11-17.

PMID: 38515630 PMC: 10953844. DOI: 10.2478/abm-2024-0003.

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