Limits of the Detection of Microplastics in Fish Tissue Using Stimulated Raman Scattering Microscopy

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2024 Mar 18

PMID 38495716

Authors

Moritz Floess

Marie Fagotto-Kaufmann

Andrea Gall

Tobias Steinle

Ingrid Ehrlich

Harald Giessen

Affiliations

Soon will be listed here.

Abstract

We demonstrate the detection sensitivity of microplastic beads within fish tissue using stimulated Raman scattering (SRS) microscopy. The intrinsically provided chemical contrast distinguishes different types of plastic compounds within fish tissue. We study the size-dependent signal-to-noise ratio of the microplastic beads and determine a lower boundary for the detectable size. Our findings demonstrate how SRS microscopy can serve as a complementary modality to conventional Raman scattering imaging in order to detect and identify microplastic particles in fish tissue.

References

Michalet X, Weiss S . Using photon statistics to boost microscopy resolution. Proc Natl Acad Sci U S A. 2006; 103(13):4797-8. PMC: 1458746. DOI: 10.1073/pnas.0600808103. View

Saar B, Freudiger C, Reichman J, Stanley C, Holtom G, Xie X . Video-rate molecular imaging in vivo with stimulated Raman scattering. Science. 2010; 330(6009):1368-70. PMC: 3462359. DOI: 10.1126/science.1197236. View

Floess M, Steinle T, Werner F, Wang Y, Wagner W, Steinle V . 3D stimulated Raman spectral imaging of water dynamics associated with pectin-glycocalyceal entanglement. Biomed Opt Express. 2023; 14(4):1460-1471. PMC: 10110326. DOI: 10.1364/BOE.485314. View

Bonfanti P, Colombo A, Saibene M, Motta G, Saliu F, Catelani T . Microplastics from miscellaneous plastic wastes: Physico-chemical characterization and impact on fish and amphibian development. Ecotoxicol Environ Saf. 2021; 225:112775. DOI: 10.1016/j.ecoenv.2021.112775. View

Shen M, Song B, Zhu Y, Zeng G, Zhang Y, Yang Y . Removal of microplastics via drinking water treatment: Current knowledge and future directions. Chemosphere. 2020; 251:126612. DOI: 10.1016/j.chemosphere.2020.126612. View

Freudiger C, Min W, Saar B, Lu S, Holtom G, He C . Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy. Science. 2008; 322(5909):1857-61. PMC: 3576036. DOI: 10.1126/science.1165758. View

Fu D, Holtom G, Freudiger C, Zhang X, Xie X . Hyperspectral imaging with stimulated Raman scattering by chirped femtosecond lasers. J Phys Chem B. 2012; 117(16):4634-40. PMC: 3637845. DOI: 10.1021/jp308938t. View

van Wezel A, Caris I, Kools S . Release of primary microplastics from consumer products to wastewater in the Netherlands. Environ Toxicol Chem. 2015; 35(7):1627-31. DOI: 10.1002/etc.3316. View

Genchi L, Martin C, Laptenok S, Baalkhuyur F, Duarte C, Liberale C . When microplastics are not plastic: Chemical characterization of environmental microfibers using stimulated Raman microspectroscopy. Sci Total Environ. 2023; 892:164671. DOI: 10.1016/j.scitotenv.2023.164671. View

10.

Maurizi L, Iordachescu L, Kirstein I, Nielsen A, Vollertsen J . Do drinking water plants retain microplastics? An exploratory study using Raman micro-spectroscopy. Heliyon. 2023; 9(6):e17113. PMC: 10361326. DOI: 10.1016/j.heliyon.2023.e17113. View

11.

Clark N, Khan F, Mitrano D, Boyle D, Thompson R . Demonstrating the translocation of nanoplastics across the fish intestine using palladium-doped polystyrene in a salmon gut-sac. Environ Int. 2021; 159:106994. DOI: 10.1016/j.envint.2021.106994. View

12.

Sheppard C . Resolution and super-resolution. Microsc Res Tech. 2017; 80(6):590-598. DOI: 10.1002/jemt.22834. View

13.

Das S, Liang Y, Tanaka S, Ozeki Y, Kao F . Synchronized subharmonic modulation in stimulated emission microscopy. Opt Express. 2019; 27(19):27159-27167. DOI: 10.1364/OE.27.027159. View

14.

Peller J, Mezyk S, Shidler S, Castleman J, Kaiser S, Faulkner R . Facile nanoplastics formation from macro and microplastics in aqueous media. Environ Pollut. 2022; 313:120171. DOI: 10.1016/j.envpol.2022.120171. View

15.

Lebreton L, Slat B, Ferrari F, Sainte-Rose B, Aitken J, Marthouse R . Evidence that the Great Pacific Garbage Patch is rapidly accumulating plastic. Sci Rep. 2018; 8(1):4666. PMC: 5864935. DOI: 10.1038/s41598-018-22939-w. View

16.

Riek C, Kocher C, Zirak P, Kolbl C, Fimpel P, Leitenstorfer A . Stimulated Raman scattering microscopy by Nyquist modulation of a two-branch ultrafast fiber source. Opt Lett. 2016; 41(16):3731-4. DOI: 10.1364/OL.41.003731. View

17.

Schymanski D, Goldbeck C, Humpf H, Furst P . Analysis of microplastics in water by micro-Raman spectroscopy: Release of plastic particles from different packaging into mineral water. Water Res. 2017; 129:154-162. DOI: 10.1016/j.watres.2017.11.011. View

18.

Li W, Tse H, Fok L . Plastic waste in the marine environment: A review of sources, occurrence and effects. Sci Total Environ. 2016; 566-567:333-349. DOI: 10.1016/j.scitotenv.2016.05.084. View

19.

Zhang K, Hamidian A, Tubic A, Zhang Y, Fang J, Wu C . Understanding plastic degradation and microplastic formation in the environment: A review. Environ Pollut. 2021; 274:116554. DOI: 10.1016/j.envpol.2021.116554. View

20.

Stapleton P . Micro- and nanoplastic transfer, accumulation, and toxicity in humans. Curr Opin Toxicol. 2021; 28:62-69. PMC: 8654083. DOI: 10.1016/j.cotox.2021.10.001. View