Resolution and Super-resolution
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Many papers have claimed the attainment of super-resolution, i.e. resolution beyond that achieved classically, by measurement of the profile of a feature in the image. We argue that measurement of the contrast of the image of a dark bar on a bright background does not give a measure of resolution, but of detection sensitivity. The width of a bar that gives an intensity at the center of the bar of 0.735 that in the bright region (the same ratio as in the Rayleigh resolution criterion) is λ/(13.9×numerical aperture) for the coherent case with central illumination. This figure, which compares with λ/(numerical aperture) for the Abbe resolution limit with central illumination, holds for the classical case, and so is no indication of super-resolution. Theoretical images for two points, two lines, arrays of lines, arrays of bars, and grating objects are compared. These results can be used a reference for experimental results, to determine if super-resolution has indeed been attained. The history of the development of the theory of microscope resolution is outlined.
Roadmap on Label-Free Super-Resolution Imaging.
Astratov V, Sahel Y, Eldar Y, Huang L, Ozcan A, Zheludev N Laser Photon Rev. 2024; 17(12).
PMID: 38883699 PMC: 11178318. DOI: 10.1002/lpor.202200029.
Floess M, Fagotto-Kaufmann M, Gall A, Steinle T, Ehrlich I, Giessen H Biomed Opt Express. 2024; 15(3):1528-1539.
PMID: 38495716 PMC: 10942679. DOI: 10.1364/BOE.519561.
Correlative nanoscopy: A multimodal approach to molecular resolution.
Jadavi S, Bianchini P, Cavalleri O, Dante S, Canale C, Diaspro A Microsc Res Tech. 2021; 84(10):2472-2482.
PMID: 33955625 PMC: 8518117. DOI: 10.1002/jemt.23800.
Super-resolution structured illumination microscopy: past, present and future.
Prakash K, Diederich B, Reichelt S, Heintzmann R, Schermelleh L Philos Trans A Math Phys Eng Sci. 2021; 379(2199):20200143.
PMID: 33896205 PMC: 8366908. DOI: 10.1098/rsta.2020.0143.
Parameter-free image resolution estimation based on decorrelation analysis.
Descloux A, Grussmayer K, Radenovic A Nat Methods. 2019; 16(9):918-924.
PMID: 31451766 DOI: 10.1038/s41592-019-0515-7.