Mechanical Stimulation of Adipose-Derived Stromal/Stem Cells for Functional Tissue Engineering of the Musculoskeletal System Via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Mar 13

PMID 38478246

Authors

Rachel C Nordberg

Josie C Bodle

Elizabeth G Loboa

Affiliations

Soon will be listed here.

Abstract

It is critical that human adipose-derived stromal/stem cell (hASC) tissue engineering therapies possess appropriate mechanical properties in order to restore the function of the load-bearing tissues of the musculoskeletal system. In an effort to elucidate hASC response to mechanical stimulation and develop mechanically robust tissue-engineered constructs, recent research has utilized a variety of mechanical loading paradigms, including cyclic tensile strain, cyclic hydrostatic pressure, and mechanical unloading in simulated microgravity. This chapter will describe the methods for applying these mechanical stimuli to hASC to direct differentiation for functional tissue engineering of the musculoskeletal system.

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