Biogenerated Oxygen-Related Environmental Stressed Apoptotic Vesicle Targets Endothelial Cells

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2024 Mar 13

PMID 38477548

Authors

Qiuyu Zhao

Bolun Lu

Shutong Qian

Jiayi Mao

Liucheng Zhang

Yuguang Zhang

Xiyuan Mao

Wenguo Cui

Xiaoming Sun

Affiliations

Soon will be listed here.

Abstract

The dynamic balance between hypoxia and oxidative stress constitutes the oxygen-related microenvironment in injured tissues. Due to variability, oxygen homeostasis is usually not a therapeutic target for injured tissues. It is found that when administered intravenously, mesenchymal stem cells (MSCs) and in vitro induced apoptotic vesicles (ApoVs) exhibit similar apoptotic markers in the wound microenvironment where hypoxia and oxidative stress co-existed, but MSCs exhibited better effects in promoting angiogenesis and wound healing. The derivation pathway of ApoVs by inducing hypoxia or oxidative stress in MSCs to simulate oxygen homeostasis in injured tissues is improved. Two types of oxygen-related environmental stressed ApoVs are identified that directly target endothelial cells (ECs) for the accurate regulation of vascularization. Compared to normoxic and hypoxic ones, oxidatively stressed ApoVs (Oxi-ApoVs) showed the strongest tube formation capacity. Different oxygen-stressed ApoVs deliver similar miRNAs, which leads to the broad upregulation of EC phosphokinase activity. Finally, local delivery of Oxi-ApoVs-loaded hydrogel microspheres promotes wound healing. Oxi-ApoV-loaded microspheres achieve controlled ApoV release, targeting ECs by reducing the consumption of inflammatory cells and adapting to the proliferative phase of wound healing. Thus, the biogenerated apoptotic vesicles responding to oxygen-related environmental stress can target ECs to promote vascularization.

Citing Articles

Apoptotic mesenchymal stem cells and their secreted apoptotic extracellular vesicles: therapeutic applications and mechanisms.

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Biosynthesis of Lysosomally Escaped Apoptotic Bodies Inhibits Inflammasome Synthesis in Macrophages.

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PMID: 39850366 PMC: 11754539. DOI: 10.34133/research.0581.

Biogenerated Oxygen-Related Environmental Stressed Apoptotic Vesicle Targets Endothelial Cells.

Zhao Q, Lu B, Qian S, Mao J, Zhang L, Zhang Y Adv Sci (Weinh). 2024; 11(20):e2306555.

PMID: 38477548 PMC: 11132028. DOI: 10.1002/advs.202306555.

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