Neuroprotective and Anti-Inflammatory Activities of Hybrid Small-Molecule SA-10 in Ischemia/Reperfusion-Induced Retinal Neuronal Injury Models

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2024 Mar 13

PMID 38474360

Authors

Charles E Amankwa

Lorea Gamboa Acha

Adnan Dibas

Sai H Chavala

Steven Roth

Biji Mathew

Suchismita Acharya

Affiliations

Soon will be listed here.

Abstract

Methods: To induce I/R injury and inflammation, we subjected R28 and primary microglial cells to oxygen glucose deprivation (OGD) for 6 h in vitro or treated these cells with a cocktail of TNF-α, IL-1β and IFN-γ for 1 h, followed by the addition of (10 µM). Inhibition of microglial activation, ROS scavenging, cytoprotective and anti-inflammatory activities were measured. In vivo I/R-injured mouse retinas were treated with either PBS or (2%) intravitreally, and pattern electroretinogram (ERG), spectral-domain optical coherence tomography, flash ERG and retinal immunocytochemistry were performed.

Results: significantly inhibited microglial activation and inflammation in vitro. Compared to the control, the compound significantly attenuated cell death in both microglia (43% vs. 13%) and R28 cells (52% vs. 17%), decreased ROS (38% vs. 68%) production in retinal microglia cells, preserved neural retinal function and increased SOD1 in mouse eyes.

Conclusion: is protective to retinal neurons by decreasing oxidative stress and inflammatory cytokines.

Citing Articles

Astragalus polysaccharide attenuates retinal ischemia reperfusion-induced microglial activation through sortilin-related vacuolar protein sorting 10 domain containing receptor 2/laminin subunit alpha 1 upregulation.

Li J, Li H, Wei C, Chen C, Zheng Z Cytojournal. 2025; 22:2.

PMID: 39958884 PMC: 11829307. DOI: 10.25259/Cytojournal_131_2024.

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