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Comprehensive Evaluation of Nucleic Acid Amplification Methods Widely Used for Generic Detection of Sandfly-borne Phleboviruses

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Specialty Microbiology
Date 2024 Mar 8
PMID 38456695
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Abstract

Importance: Virus nucleic acid testing is the primary diagnostic method, particularly in the early stages of illness. Virus-specific or syndromic tests are widely used for this purpose. The use of generic primers has had a considerable impact on the discovery, identification, and detection of Old World sandfly-borne phleboviruses (OWSBP). The study is significant because it is the first to carry out a comparative evaluation of all published OWSBP generic primer sets.

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