A Lipo-polymeric Hybrid Nanosystem with Metal Enhanced Fluorescence for Targeted Imaging of Metastatic Breast Cancer

Overview

Journal Nanotheranostics

Specialty Biotechnology

Date 2024 Mar 6

PMID 38444742

Authors

Tejaswini Appidi

Rajalakshmi P Sivasankaran

Shubham A Chinchulkar

Paloma Patra

Kavipriya Murugaiyan

Bantal Veeresh

Aravind Kumar Rengan

Affiliations

Soon will be listed here.

Abstract

Cancer metastasis plays a major role in failure of therapeutic avenues against cancer. Owing to metastasis, nearly 70-80% of stage IV breast cancer patients lose their lives. Nanodrug delivery systems are playing a critical role in the therapy of metastatic cancer in the recent times. This paper reports the enhanced permeation and retention (EPR) based targeting of metastatic breast cancer using a novel nano lipo-polymeric system (PIR-Au NPs). The PIR-Au NPs demonstrated an increase in fluorescence by virtue of surface coating with gold, owing to the metal enhanced fluorescence phenomenon as reported in our earlier reports. Enhanced fluorescence of PIR-Au NPs was observed in murine mammary carcinoma cell line (4T1), as compared to free IR780 or IR780 loaded nanosystems (P-IR NPs), when incubated for same time at same concentrations, indicating its potential application for imaging and an enhanced bioavailability of IR780. Significant cell death was noted with photothermal mediated cytotoxicity against breast cancer cells (MCF-7 and 4T1). An enhanced fluorescence was observed in the zebra fish embryos incubated with PIR-Au NPs. The enhanced permeation and retention (EPR) effect was seen with PIR-Au NPs . A strong fluorescent signal was recorded in mice injected with PIR-Au NPs. The tumor tissue collected after 72 h, clearly showed a greater fluorescence as compared to other groups, indicating the plasmon enhanced fluorescence. We also demonstrated the EPR-based targeting of the PIR-Au NPs by means of photothermal heat This lipo-polymeric hybrid nanosystem could therefore be successfully applied for image-guided, passive-targeting to achieve maximum therapeutic benefits.

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References

Sun H, Su J, Meng Q, Yin Q, Chen L, Gu W . Cancer-Cell-Biomimetic Nanoparticles for Targeted Therapy of Homotypic Tumors. Adv Mater. 2016; 28(43):9581-9588. DOI: 10.1002/adma.201602173. View

You P, Li F, Liu M, Chan Y . Colorimetric and Fluorescent Dual-Mode Immunoassay Based on Plasmon-Enhanced Fluorescence of Polymer Dots for Detection of PSA in Whole Blood. ACS Appl Mater Interfaces. 2019; 11(10):9841-9849. DOI: 10.1021/acsami.9b00204. View

Chen C, Tang W, Jiang D, Yang G, Wang X, Zhou L . Hyaluronic acid conjugated polydopamine functionalized mesoporous silica nanoparticles for synergistic targeted chemo-photothermal therapy. Nanoscale. 2019; 11(22):11012-11024. DOI: 10.1039/c9nr01385g. View

Chen Q, Wen J, Li H, Xu Y, Liu F, Sun S . Recent advances in different modal imaging-guided photothermal therapy. Biomaterials. 2016; 106:144-66. DOI: 10.1016/j.biomaterials.2016.08.022. View

Wang L, Niu C . IR780-based nanomaterials for cancer imaging and therapy. J Mater Chem B. 2021; 9(20):4079-4097. DOI: 10.1039/d1tb00407g. View

Kim H, Kang M, Choo Y, Go S, Kwon S, Song S . Immunomodulatory Lipocomplex Functionalized with Photosensitizer-Embedded Cancer Cell Membrane Inhibits Tumor Growth and Metastasis. Nano Lett. 2019; 19(8):5185-5193. DOI: 10.1021/acs.nanolett.9b01571. View

Wang Y, Liu T, Zhang E, Luo S, Tan X, Shi C . Preferential accumulation of the near infrared heptamethine dye IR-780 in the mitochondria of drug-resistant lung cancer cells. Biomaterials. 2014; 35(13):4116-24. DOI: 10.1016/j.biomaterials.2014.01.061. View

Jiang C, Cheng H, Yuan A, Tang X, Wu J, Hu Y . Hydrophobic IR780 encapsulated in biodegradable human serum albumin nanoparticles for photothermal and photodynamic therapy. Acta Biomater. 2014; 14:61-9. DOI: 10.1016/j.actbio.2014.11.041. View

Iyer A, Khaled G, Fang J, Maeda H . Exploiting the enhanced permeability and retention effect for tumor targeting. Drug Discov Today. 2006; 11(17-18):812-8. DOI: 10.1016/j.drudis.2006.07.005. View

10.

Appidi T, Rajalakshmi P S , Chinchulkar S, Pradhan A, Begum H, Shetty V . A plasmon-enhanced fluorescent gold coated novel lipo-polymeric hybrid nanosystem: synthesis, characterization and application for imaging and photothermal therapy of breast cancer. Nanoscale. 2022; 14(25):9112-9123. DOI: 10.1039/d2nr01378a. View

11.

Song J, Zhang N, Zhang L, Yi H, Liu Y, Li Y . IR780-loaded folate-targeted nanoparticles for near-infrared fluorescence image-guided surgery and photothermal therapy in ovarian cancer. Int J Nanomedicine. 2019; 14:2757-2772. PMC: 6503636. DOI: 10.2147/IJN.S203108. View

12.

Lin C, Huang S, Colangelo M, Chen C, Wong F, He Y . Surface Plasmon Enhanced Upconversion Fluorescence in Short-Wave Infrared for In Vivo Imaging of Ovarian Cancer. ACS Nano. 2022; 16(8):12930-12940. DOI: 10.1021/acsnano.2c05301. View

13.

Machado M, de Oliveira M, Lanna E, Siqueira R, Pound-Lana G, Branquinho R . Photodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells. Biomed Pharmacother. 2021; 145:112464. DOI: 10.1016/j.biopha.2021.112464. View

14.

Xie W, Deng W, Zan M, Rao L, Yu G, Zhu D . Cancer Cell Membrane Camouflaged Nanoparticles to Realize Starvation Therapy Together with Checkpoint Blockades for Enhancing Cancer Therapy. ACS Nano. 2019; 13(3):2849-2857. DOI: 10.1021/acsnano.8b03788. View

15.

Sung H, Ferlay J, Siegel R, Laversanne M, Soerjomataram I, Jemal A . Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021; 71(3):209-249. DOI: 10.3322/caac.21660. View

16.

Yuan A, Wu J, Tang X, Zhao L, Xu F, Hu Y . Application of near-infrared dyes for tumor imaging, photothermal, and photodynamic therapies. J Pharm Sci. 2012; 102(1):6-28. DOI: 10.1002/jps.23356. View

17.

Ye J, Fu G, Yan X, Liu J, Wang X, Cheng L . Noninvasive magnetic resonance/photoacoustic imaging for photothermal therapy response monitoring. Nanoscale. 2018; 10(13):5864-5868. DOI: 10.1039/C8NR00044A. View

18.

Zou L, Wang H, He B, Zeng L, Tan T, Cao H . Current Approaches of Photothermal Therapy in Treating Cancer Metastasis with Nanotherapeutics. Theranostics. 2016; 6(6):762-72. PMC: 4860886. DOI: 10.7150/thno.14988. View

19.

Zhang C, Liu T, Su Y, Luo S, Zhu Y, Tan X . A near-infrared fluorescent heptamethine indocyanine dye with preferential tumor accumulation for in vivo imaging. Biomaterials. 2010; 31(25):6612-7. DOI: 10.1016/j.biomaterials.2010.05.007. View

20.

Zhang W, Ding M, Zhang H, Shang H, Zhang A . Tumor acidity and near-infrared light responsive drug delivery MoS-based nanoparticles for chemo-photothermal therapy. Photodiagnosis Photodyn Ther. 2022; 38:102716. DOI: 10.1016/j.pdpdt.2022.102716. View