The Copy Number of the Eukaryotic RRNA Gene Can Be Counted Comprehensively

Overview

Journal Microbiologyopen

Specialty Microbiology

Date 2024 Mar 4

PMID 38436548

Authors

Akinori Yabuki

Tatsuhiko Hoshino

Tamiko Nakamura

Keiko Mizuno

Affiliations

Soon will be listed here.

Abstract

Gene sequence has been widely used in molecular ecology. For instance, the ribosomal RNA (rRNA) gene has been widely used as a biological marker to understand microbial communities. The variety of the detected rRNA gene sequences reflects the diversity of the microorganisms existing in the analyzed sample. Their biomass can also be estimated by applying quantitative sequencing with information on rRNA gene copy numbers in genomes; however, information on rRNA gene copy numbers is still limited. Especially, the copy number in microbial eukaryotes is much less understood than that of prokaryotes, possibly because of the large and complex structure of eukaryotic genomes. In this study, we report an alternative approach that is more appropriate than the existing method of quantitative sequencing and demonstrate that the copy number of eukaryotic rRNA can be measured efficiently and comprehensively. By applying this approach widely, information on the eukaryotic rRNA copy number can be determined, and their community structures can be depicted and compared more efficiently.

Citing Articles

The copy number of the eukaryotic rRNA gene can be counted comprehensively.

Yabuki A, Hoshino T, Nakamura T, Mizuno K Microbiologyopen. 2024; 13(2):e1399.

PMID: 38436548 PMC: 10910464. DOI: 10.1002/mbo3.1399.

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