Structure of the MalB Region in Escherichia Coli K12. I. Genetic Map of the MalK-lamB Operon

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1979 Jul 24

PMID 384166

Citations 57

Authors

O Raibaud

M Roa

M Schwartz

Affiliations

Soon will be listed here.

Abstract

A series of deletions, Mu insertions and point mutations affecting the malK-lamB operon have been isolated. They were used to establish a deletion map of this operon, which could be divided in 27 intervals, with 16 in malK and 11 in lamB. One interesting feature of this map is the lack of randomness in the distribution of Mu insertions in the lamB gene; by using data published elsewhere on the physical length of the deletion intervals it can be concluded that about 25% of these Mu insertions are clustered in a segment representing 2 to 8% of the gene. This map is presently being used to study the biosynthesis, structure, and function of the lamB product, which is an outer membrane protein involved in the transport of maltose and maltodextrin, and which in addition constitutes the receptor for phage lambda.

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