Obacunone Represses Salmonella Pathogenicity Islands 1 and 2 in an EnvZ-dependent Fashion

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2012 Jul 31

PMID 22843534

Citations 13

Authors

Amit Vikram

Guddadarangavvanahally K Jayaprakasha

Palmy R Jesudhasan

Suresh D Pillai

Bhimanagouda S Patil

Affiliations

Soon will be listed here.

Abstract

Obacunone belongs to a class of unique triterpenoids called limonoids, present in Citrus species. Previous studies from our laboratory suggested that obacunone possesses antivirulence activity and demonstrates inhibition of cell-cell signaling in Vibrio harveyi and Escherichia coli O157:H7. The present work sought to determine the effect of obacunone on the food-borne pathogen Salmonella enterica serovar Typhimurium LT2 by using a cDNA microarray. Transcriptomic studies indicated that obacunone represses Salmonella pathogenicity island 1 (SPI1), the maltose transporter, and the hydrogenase operon. Furthermore, phenotypic data for the Caco-2 infection assay and maltose utilization were in agreement with microarray data suggesting repression of SPI1 and maltose transport. Further studies demonstrated that repression of SPI1 was plausibly mediated through hilA. Additionally, obacunone seems to repress SPI2 under SPI2-inducing conditions as well as in Caco-2 infection models. Furthermore, obacunone seems to repress hilA in an EnvZ-dependent fashion. Altogether, the results of the study seems to suggest that obacunone exerts an antivirulence effect on S. Typhimurium and may serve as a lead compound for development of antivirulence strategies for S. Typhimurium.

Citing Articles

Obacunone, a Promising Phytochemical Triterpenoid: Research Progress on Its Pharmacological Activity and Mechanism.

Zhou Y, Gu J, Li J, Zhang H, Wang M, Li Y Molecules. 2024; 29(8).

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Nomilin and Its Analogues in Citrus Fruits: A Review of Its Health Promotion Effects and Potential Application in Medicine.

Zhou Z, Yan Y, Li H, Feng Y, Huang C, Fan S Molecules. 2023; 28(1).

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Obacunone alleviates ferroptosis during lipopolysaccharide-induced acute lung injury by upregulating Nrf2-dependent antioxidant responses.

Li J, Deng S, Li J, Li L, Zhang F, Zou Y Cell Mol Biol Lett. 2022; 27(1):29.

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G-quadruplex stabilization in the ions and maltose transporters gene inhibit Salmonella enterica growth and virulence.

Jain N, Mishra S, Shankar U, Jaiswal A, Sharma T, Kodgire P Genomics. 2020; 112(6):4863-4874.

PMID: 32898642 PMC: 7474834. DOI: 10.1016/j.ygeno.2020.09.010.

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