Make It Shine: Labelling the ER for Light and Fluorescence Microscopy
Overview
Overview
Authors
Affiliations
Affiliations
Soon will be listed here.
Abstract
The ER is a highly dynamic network of tubules and membrane cisternae. Hence, imaging this organelle in its native and mobile state is of great importance. Here we describe methods of labelling the native plant ER using fluorescent proteins and lipid dyes as well as methods for immunolabelling on plant tissue.
Citing Articles
McGinness A, Brooks S, Strasser R, Schoberer J, Kriechbaumer V J Microsc. 2024; 297(3):258-273.
PMID: 38687244 PMC: 11808412. DOI: 10.1111/jmi.13311.
References
1.
Wang P, Hawkins T, Richardson C, Cummins I, Deeks M, Sparkes I
. The plant cytoskeleton, NET3C, and VAP27 mediate the link between the plasma membrane and endoplasmic reticulum. Curr Biol. 2014; 24(12):1397-1405.
DOI: 10.1016/j.cub.2014.05.003.
View
2.
Terasaki M, Song J, Wong J, Weiss M, Chen L
. Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes. Cell. 1984; 38(1):101-8.
DOI: 10.1016/0092-8674(84)90530-0.
View
3.
Sparkes I, Tolley N, Aller I, Svozil J, Osterrieder A, Botchway S
. Five Arabidopsis reticulon isoforms share endoplasmic reticulum location, topology, and membrane-shaping properties. Plant Cell. 2010; 22(4):1333-43.
PMC: 2879755.
DOI: 10.1105/tpc.110.074385.
View
4.
Huang L, Franklin A, Hoffman N
. Primary structure and characterization of an Arabidopsis thaliana calnexin-like protein. J Biol Chem. 1993; 268(9):6560-6.
View
5.
Ivanov S, Harrison M
. A set of fluorescent protein-based markers expressed from constitutive and arbuscular mycorrhiza-inducible promoters to label organelles, membranes and cytoskeletal elements in Medicago truncatula. Plant J. 2014; 80(6):1151-63.
DOI: 10.1111/tpj.12706.
View