Characterization of the Newborn Epidermis and Adult Hair Follicles Using Whole-Mount Immunofluorescent Staining of Mouse Dorsal Skin

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Feb 26

PMID 38407797

Authors

Gaia Cangiotti

Anthony Veltri

Georges Delepine

Wen-Hui Lien

Affiliations

Soon will be listed here.

Abstract

The mammalian integumentary system, including skin and its appendages, serves as a protective barrier for the body. During development, skin epidermis undergoes rapid cell division and differentiation to form multiple stratified layers of keratinocytes. Concurrently the epidermis also gives rise to hair follicles that invaginate into the dermis. In adult skin, the hair follicle undergoes cyclic regeneration fueled by hair follicle stem cells located in the bulge. Three-dimensional and high-resolution imaging of these structures using whole-mount immunofluorescent staining allows to better characterize epidermal progenitors and stem cells.

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