Polymorphisms in Innate and Adaptive Immune Genes in Subjects with Allergic Bronchopulmonary Aspergillosis Complicating Asthma

Overview

Journal Mycopathologia

Specialties Microbiology
Pharmacology

Date 2024 Feb 26

PMID 38407762

Authors

Rimjhim Kanaujia

Amit Arora

Arunaloke Chakrabarti

Shivaprakash M Rudramurthy

Ritesh Agarwal

Affiliations

Soon will be listed here.

Abstract

Innate and adaptive immunity play a crucial role in allergic bronchopulmonary aspergillosis (ABPA) pathogenesis. We performed next-generation sequencing using the Illumina TruSight One panel (4,811 human disease-associated genes, at least 20 × coverage) and selected 22 known immune genes (toll-like receptors (TLRs), C-type lectin, interleukin-4 receptor, and others). We included ABPA (n = 18), asthma without ABPA (n = 12), and healthy controls (n = 8). We analyzed 3011 SNPs from 22 genes and identified 145 SNPs (13 genes) that were present only in the disease groups and absent in controls. The SNP frequency overall was significantly higher in ABPA than in asthmatics (89/145 [61.4%] vs. 56/145 [38.6%], p = 0.0001). The SNP frequency in the TLR10 gene was also significantly higher in ABPA than in asthma (p = 0.017). Association analysis further revealed three genes having significant associations. Of these, NOS3 and HLA-DQB1 are associated with antimicrobial activity and adaptive immunity. More extensive studies are required to confirm our findings.

Citing Articles

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Jaggi T, Agarwal R, Tiew P, Shah A, Lydon E, Hage C Eur Respir J. 2024; 64(5).

PMID: 39362667 PMC: 11602666. DOI: 10.1183/13993003.00803-2024.

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