Cheese Containing Probiotic NJ42 Isolated from Stingless Bee Honey Reduces Weight Gain, Fat Accumulation, and Glucose Intolerance in Mice

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Feb 26

PMID 38404857

Authors

Nor Hazwani Mohd Hasali

Amir Izzwan Zamri

Mohd Nizam Lani

Vance Matthews

Aidilla Mubarak

Affiliations

Soon will be listed here.

Abstract

Background: The high occurrence of metabolic syndrome has driven a growing demand for natural resource-based therapeutic strategies, highlighting their potential efficacy in addressing the complexities of this condition. Probiotics are established to be useful in the prevention and treatment of diabetes and obesity. However, limited exploration exists regarding the application of the isolated strain from stingless bee honey as a probiotic within dairy products, such as cheese. This study investigated the effect of a high-fat diet supplemented with cheese containing probiotic bacteria ( strain NJ42) isolated from honey (PCHFD) on the symptoms of metabolic disorder in C57BL/6 mice.

Methods And Results: Body weight, glucose intolerance, insulin resistance, and fat accumulation were measured during 12 weeks of feeding and compared to mice fed with a normal chow (NC) and high-fat diet (HFD). Over a 12-week feeding period, PCHFD-fed mice exhibited substantial reductions in several metabolic syndrome-associated features. They had a lower rate of weight gain (p = 0.03) than the HFD-fed mice. Additionally, they displayed a notable 39.2% decrease in gonadal fat mass compared to HFD-fed mice (p = 0.003). HFD-fed mice showed impaired glucose tolerance when compared to NC-fed mice (p = 0.00). Conversely, PCHFD-fed mice showed a reduction in glucose intolerance to a level close to that of the NC-fed mice group (p = 0.01). These positive effects extended to reductions in hepatic steatosis and adipocyte hypertrophy.

Conclusion: These results indicated that strain NJ42, isolated from honey, is a prospective probiotic to lower the risk of developing metabolic syndrome features induced by a high-fat diet. These positive findings suggest the prospect of enriching commonly consumed dietary components such as cheese with probiotic attributes, potentially offering an accessible means to alleviating the symptoms of metabolic diseases.

Citing Articles

069 and 031: Unraveling Strain-Specific Pathways for Modulating Lipid Metabolism and Attenuating High-Fat-Diet-Induced Obesity in Mice.

Ho P, Chou Y, Koh Y, Lin W, Chen W, Tseng A ACS Omega. 2024; 9(26):28520-28533.

PMID: 38973907 PMC: 11223209. DOI: 10.1021/acsomega.4c02514.

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