Influence of Ultrasound Stimulation on the Viability, Proliferation and Protein Expression of Osteoblasts and Periodontal Ligament Fibroblasts

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2024 Feb 24

PMID 38397963

Authors

Selma Pascoal

Sofia Oliveira

Francisca Monteiro

Jorge Padrao

Rita Costa

Andrea Zille

Susana O Catarino

Filipe S Silva

Teresa Pinho

Oscar Carvalho

Affiliations

Soon will be listed here.

Abstract

Among the adjunctive procedures to accelerate orthodontic tooth movement (OTM), ultrasound (US) is a nonsurgical form of mechanical stimulus that has been explored as an alternative to the currently available treatments. This study aimed to clarify the role of US in OTM by exploring different stimulation parameters and their effects on the biological responses of cells involved in OTM. Human fetal osteoblasts and periodontal ligament fibroblasts cell lines were stimulated with US at 1.0 and 1.5 MHz central frequencies and power densities of 30 and 60 mW/cm in continuous mode for 5 and 10 min. Cellular proliferation, metabolic activity and protein expression were analyzed. The US parameters that significantly improved the metabolic activity were 1.0 MHz at 30 mW/cm for 5 min and 1.0 MHz at 60 mW/cm for 5 and 10 min for osteoblasts; and 1.0 MHz at 30 mW/cm for 5 min and 1.5 MHz at 60 mW/cm for 5 and 10 min for fibroblasts. By stimulating with these parameters, the expression of alkaline phosphatase was maintained, while osteoprotegerin synthesis was induced after three days of US stimulation. The US stimulation improved the biological activity of both osteoblasts and periodontal ligament fibroblasts, inducing their osteogenic differentiation.

Citing Articles

Effects of Vibration on Accelerating Orthodontic Tooth Movement in Clinical and In Vivo Studies: A Systematic Review.

Pascoal S, Oliveira S, Ascione M, Pereira J, Carvalho O, Pinho T Dent J (Basel). 2024; 12(8).

PMID: 39195087 PMC: 11353850. DOI: 10.3390/dj12080243.

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