Whole-Mount Immunofluorescence Staining to Visualize Cell Cycle Progression in Mouse Oocyte Meiosis

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Feb 23

PMID 38393478

Authors

Safia El Jailani

Katja Wassmann

Sandra A Touati

Affiliations

Soon will be listed here.

Abstract

Whole-mount immunofluorescence allows direct visualization of the cellular architecture within cells. Here, we apply this technique to mouse oocytes to visualize spindle morphology and microtubule attachments to kinetochores, using a technique we call "cold treatment," at various phases of the meiotic cell cycle. This method allows the analysis of spindle structures at different meiosis I stages and at metaphase II. An adaptation of the protocol to the cell cycle stage of interest is described.

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