Time-dependent Changes in Retinoids Content in Liver and Adipose Tissue After Feeding of a Vitamin A-deficient Diet to Mice

Overview

Journal Exp Anim

Date 2024 Feb 21

PMID 38382988

Authors

Mira Kato-Suzuki

Yuko Okamatsu-Ogura

Osamu Inanami

Kazuhiro Kimura

Affiliations

Soon will be listed here.

Abstract

Vitamin A is an important nutrient for multiple physiological functions. To elucidate the role of vitamin A in vivo, vitamin A-deficient diets have been often used in mice to establish a vitamin A-deficiency model. However, the information on the appropriate feeding periods and time course of changes in vitamin A content in organs after the start of vitamin A-deficient diet feeding is lacking. This study aimed to assess the retinoids levels in liver and white adipose tissue in mice fed a vitamin A-deficient diet for ≤8 weeks. High-performance liquid chromatography was used to measure the retinoids levels in liver and white adipose tissue every 2 weeks for ≤8 weeks. Vitamin A-deficient diet feeding significantly decreased retinol in the liver over 6 weeks, but retinyl palmitate, a main storage form of vitamin A, was not changed over 8 weeks. The plasma retinol level remained constant throughout the experiment. In white adipose tissue, retinyl palmitate gradually decreased over 8 weeks. These results indicate that vitamin A-deficient diet feeding longer than 6 weeks reduced retinol in liver and retinyl palmitate in white adipose tissue over 8 weeks, although it is not enough for the induction of a whole-body vitamin A deficiency.

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References

OByrne S, Blaner W . Retinol and retinyl esters: biochemistry and physiology. J Lipid Res. 2013; 54(7):1731-43. PMC: 3679378. DOI: 10.1194/jlr.R037648. View

Liu L, Tang X, Gudas L . Homeostasis of retinol in lecithin: retinol acyltransferase gene knockout mice fed a high retinol diet. Biochem Pharmacol. 2008; 75(12):2316-24. PMC: 2486431. DOI: 10.1016/j.bcp.2008.03.012. View

Saari J . Vitamin A and Vision. Subcell Biochem. 2016; 81:231-259. DOI: 10.1007/978-94-024-0945-1_9. View

Kim Y, Quadro L . Reverse-phase high-performance liquid chromatography (HPLC) analysis of retinol and retinyl esters in mouse serum and tissues. Methods Mol Biol. 2010; 652:263-75. PMC: 3716261. DOI: 10.1007/978-1-60327-325-1_15. View

Ross A . Diet in vitamin A research. Methods Mol Biol. 2010; 652:295-313. PMC: 3873196. DOI: 10.1007/978-1-60327-325-1_17. View

Villarroya F, Giralt M, Iglesias R . Retinoids and adipose tissues: metabolism, cell differentiation and gene expression. Int J Obes Relat Metab Disord. 1999; 23(1):1-6. DOI: 10.1038/sj.ijo.0800799. View

Blaner W, Obunike J, Kurlandsky S, Piantedosi R, Deckelbaum R, Goldberg I . Lipoprotein lipase hydrolysis of retinyl ester. Possible implications for retinoid uptake by cells. J Biol Chem. 1994; 269(24):16559-65. View

Maguire M, Bushkofsky J, Larsen M, Foong Y, Tanumihardjo S, Jefcoate C . Diet-dependent retinoid effects on liver gene expression include stellate and inflammation markers and parallel effects of the nuclear repressor Shp. J Nutr Biochem. 2017; 47:63-74. PMC: 5953423. DOI: 10.1016/j.jnutbio.2017.04.009. View

Kane M, Folias A, Napoli J . HPLC/UV quantitation of retinal, retinol, and retinyl esters in serum and tissues. Anal Biochem. 2008; 378(1):71-9. PMC: 2483537. DOI: 10.1016/j.ab.2008.03.038. View

10.

Oliveros L, Domeniconi M, Vega V, Gatica L, Brigada A, Gimenez M . Vitamin A deficiency modifies lipid metabolism in rat liver. Br J Nutr. 2007; 97(2):263-72. DOI: 10.1017/S0007114507182659. View

11.

Haaker M, Vaandrager A, Helms J . Retinoids in health and disease: A role for hepatic stellate cells in affecting retinoid levels. Biochim Biophys Acta Mol Cell Biol Lipids. 2020; 1865(6):158674. DOI: 10.1016/j.bbalip.2020.158674. View

12.

Frey S, Vogel S . Vitamin A metabolism and adipose tissue biology. Nutrients. 2012; 3(1):27-39. PMC: 3257733. DOI: 10.3390/nu3010027. View

13.

Zhou Y, Zhou J, Zhang Y, Tang J, Sun B, Xu W . Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency. J Diabetes Res. 2020; 2020:2354108. PMC: 6996671. DOI: 10.1155/2020/2354108. View

14.

Niederreither K, Dolle P . Retinoic acid in development: towards an integrated view. Nat Rev Genet. 2008; 9(7):541-53. DOI: 10.1038/nrg2340. View

15.

Vogel S, Piantedosi R, OByrne S, Kako Y, Quadro L, Gottesman M . Retinol-binding protein-deficient mice: biochemical basis for impaired vision. Biochemistry. 2002; 41(51):15360-8. DOI: 10.1021/bi0268551. View

16.

Blomhoff R, Blomhoff H . Overview of retinoid metabolism and function. J Neurobiol. 2006; 66(7):606-30. DOI: 10.1002/neu.20242. View

17.

Baleato R, Aitken R, Roman S . Vitamin A regulation of BMP4 expression in the male germ line. Dev Biol. 2005; 286(1):78-90. DOI: 10.1016/j.ydbio.2005.07.009. View

18.

BARUA A, Olson J . Reversed-phase gradient high-performance liquid chromatographic procedure for simultaneous analysis of very polar to nonpolar retinoids, carotenoids and tocopherols in animal and plant samples. J Chromatogr B Biomed Sci Appl. 1998; 707(1-2):69-79. DOI: 10.1016/s0378-4347(97)00614-2. View

19.

Oliveira L, Emidio Teixeira F, Sato M . Impact of Retinoic Acid on Immune Cells and Inflammatory Diseases. Mediators Inflamm. 2018; 2018:3067126. PMC: 6109577. DOI: 10.1155/2018/3067126. View

20.

Clagett-Dame M, Deluca H . The role of vitamin A in mammalian reproduction and embryonic development. Annu Rev Nutr. 2002; 22:347-81. DOI: 10.1146/annurev.nutr.22.010402.102745E. View