Genome-wide Analysis of BZIP Gene Family Members in Pleurotus Ostreatus, and Potential Roles of PobZIP3 in Development and the Heat Stress Response

Overview

Journal Microb Biotechnol

Specialties Biotechnology
Microbiology

Date 2024 Feb 20

PMID 38376071

Authors

Yuanyuan Zhou

Zihao Li

Congtao Xu

Jinlong Pan

Haikang Li

Yi Zhou

Yajie Zou

Affiliations

Soon will be listed here.

Abstract

The basic leucine zipper (bZIP) transcription factor (TF) is widespread among eukaryotes and serves different roles in fungal processes including nutrient utilization, growth, stress responses and development. The oyster mushroom (Pleurotus ostreatus) is an important and widely cultivated edible mushroom worldwide; nevertheless, reports are lacking on the identification or function of bZIP gene family members in P. ostreatus. Herein, 11 bZIPs on 6 P. ostreatus chromosomes were systematically identified, which were classified into 3 types according to their protein sequences. Phylogenetic analysis of PobZIPs with other fungal bZIPs indicated that PobZIPs may have differentiated late. Cis-regulatory element analysis revealed that at least one type of stress-response-related element was present on each bZIP promoter. RNA-seq and RT-qPCR analyses revealed that bZIP expression patterns were altered under heat stress and different developmental stages. We combined results from GST-Pull-down, EMSA and yeast two-hybrid assays to screen a key heat stress-responsive candidate gene PobZIP3. PobZIP3 overexpression in P. ostreatus enhanced tolerance to high temperature and cultivation assays revealed that PobZIP3 positively regulates the development of P. ostreatus. RNA-seq analysis showed that PobZIP3 plays a role in glucose metabolism pathways, antioxidant enzyme activity and sexual reproduction. These results may support future functional studies of oyster mushroom bZIP TFs.

Citing Articles

Genome-Wide Profiling of bZIP Transcription Factors and FocbZIP11's Impact on Fusarium TR4 Pathogenicity.

Xie Y, Huang H, Huo Y, Yang W, Li Y, Liu S Int J Mol Sci. 2025; 26(4).

PMID: 40003918 PMC: 11855148. DOI: 10.3390/ijms26041452.

Genome-wide analysis of bZIP gene family members in Pleurotus ostreatus, and potential roles of PobZIP3 in development and the heat stress response.

Zhou Y, Li Z, Xu C, Pan J, Li H, Zhou Y Microb Biotechnol. 2024; 17(2):e14413.

PMID: 38376071 PMC: 10877997. DOI: 10.1111/1751-7915.14413.

References

Hou Z, Chen Q, Zhao M, Huang C, Wu X . Genome-wide characterization of the Zn(II)Cys zinc cluster-encoding gene family in and expression analyses of this family during developmental stages and under heat stress. PeerJ. 2020; 8:e9336. PMC: 7295025. DOI: 10.7717/peerj.9336. View

Wang L, Wu X, Gao W, Zhao M, Zhang J, Huang C . Differential Expression Patterns of Pleurotus ostreatus Catalase Genes during Developmental Stages and under Heat Stress. Genes (Basel). 2017; 8(11). PMC: 5704248. DOI: 10.3390/genes8110335. View

Chen C, Chen H, Zhang Y, Thomas H, Frank M, He Y . TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data. Mol Plant. 2020; 13(8):1194-1202. DOI: 10.1016/j.molp.2020.06.009. View

Nijhawan A, Jain M, Tyagi A, Khurana J . Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice. Plant Physiol. 2007; 146(2):333-50. PMC: 2245831. DOI: 10.1104/pp.107.112821. View

Lu M, Meng X, Zhang Y, Zhu X, Li J, Chen W . Genome-Wide Identification and Expression Profiles of Genes in L. Cannabis Cannabinoid Res. 2022; 7(6):882-895. DOI: 10.1089/can.2021.0153. View

Ma H, Liu C, Li Z, Ran Q, Xie G, Wang B . ZmbZIP4 Contributes to Stress Resistance in Maize by Regulating ABA Synthesis and Root Development. Plant Physiol. 2018; 178(2):753-770. PMC: 6181033. DOI: 10.1104/pp.18.00436. View

Gao J, Davidson M, Wahls W . Distinct regions of ATF/CREB proteins Atf1 and Pcr1 control recombination hotspot ade6-M26 and the osmotic stress response. Nucleic Acids Res. 2008; 36(9):2838-51. PMC: 2396409. DOI: 10.1093/nar/gkn037. View

Zou Y, Zhang M, Qu J, Zhang J . iTRAQ-Based Quantitative Proteomic Analysis Reveals Proteomic Changes in Mycelium of in Response to Heat Stress and Subsequent Recovery. Front Microbiol. 2018; 9:2368. PMC: 6189471. DOI: 10.3389/fmicb.2018.02368. View

Shin J, Bui D, Kim S, Jung S, Nam H, Lim J . The novel bZIP transcription factor Fpo1 negatively regulates perithecial development by modulating carbon metabolism in the ascomycete fungus Fusarium graminearum. Environ Microbiol. 2020; 22(7):2596-2612. DOI: 10.1111/1462-2920.14960. View

10.

He G, Xu J, Wang Y, Liu J, Li P, Chen M . Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis. BMC Plant Biol. 2016; 16(1):116. PMC: 4877946. DOI: 10.1186/s12870-016-0806-4. View

11.

Qu D, Wu F, Zhao X, Zhu D, Gu L, Yang L . A bZIP transcription factor VabZIP12 from blueberry induced by dark septate endocyte improving the salt tolerance of transgenic Arabidopsis. Plant Sci. 2022; 315:111135. DOI: 10.1016/j.plantsci.2021.111135. View

12.

Shelest E . Transcription factors in fungi. FEMS Microbiol Lett. 2008; 286(2):145-51. DOI: 10.1111/j.1574-6968.2008.01293.x. View

13.

Lei M, Wu X, Huang C, Qiu Z, Wang L, Zhang R . Trehalose induced by reactive oxygen species relieved the radial growth defects of Pleurotus ostreatus under heat stress. Appl Microbiol Biotechnol. 2019; 103(13):5379-5390. DOI: 10.1007/s00253-019-09834-8. View

14.

Son H, Seo Y, Min K, Park A, Lee J, Jin J . A phenome-based functional analysis of transcription factors in the cereal head blight fungus, Fusarium graminearum. PLoS Pathog. 2011; 7(10):e1002310. PMC: 3197617. DOI: 10.1371/journal.ppat.1002310. View

15.

Wang W, Wang Y, Zhang S, Xie K, Zhang C, Xi Y . Genome-wide analysis of the abiotic stress-related bZIP family in switchgrass. Mol Biol Rep. 2020; 47(6):4439-4454. DOI: 10.1007/s11033-020-05561-w. View

16.

Hou L, Zhao M, Huang C, He Q, Zhang L, Zhang J . Alternative oxidase gene induced by nitric oxide is involved in the regulation of ROS and enhances the resistance of Pleurotus ostreatus to heat stress. Microb Cell Fact. 2021; 20(1):137. PMC: 8287771. DOI: 10.1186/s12934-021-01626-y. View

17.

Dong Y, Zhang J, Gao Z, Zhao H, Sun G, Wang X . Characterization and anti-hyperlipidemia effects of enzymatic residue polysaccharides from Pleurotus ostreatus. Int J Biol Macromol. 2019; 129:316-325. DOI: 10.1016/j.ijbiomac.2019.01.164. View

18.

Zong W, Tang N, Yang J, Peng L, Ma S, Xu Y . Feedback Regulation of ABA Signaling and Biosynthesis by a bZIP Transcription Factor Targets Drought-Resistance-Related Genes. Plant Physiol. 2016; 171(4):2810-25. PMC: 4972276. DOI: 10.1104/pp.16.00469. View

19.

Li F, Liu J, Guo X, Yin L, Zhang H, Wen R . Genome-wide survey, characterization, and expression analysis of bZIP transcription factors in Chenopodium quinoa. BMC Plant Biol. 2020; 20(1):405. PMC: 7466520. DOI: 10.1186/s12870-020-02620-z. View

20.

Schutze K, Harter K, Chaban C . Post-translational regulation of plant bZIP factors. Trends Plant Sci. 2008; 13(5):247-55. DOI: 10.1016/j.tplants.2008.03.002. View