Chromatograms and Mass Spectra of High-Mannose and Paucimannose -Glycans for Rapid Isomeric Identifications

Overview

Journal J Proteome Res

Publisher American Chemical Society

Specialty Biochemistry

Date 2024 Feb 16

PMID 38364797

Authors

Chia Yen Liew

Jien-Lian Chen

Yen-Ting Lin

Hong-Sheng Luo

An-Ti Hung

Bryan John Abel Magoling

Hock-Seng Nguan

Charles Pin-Kuang Lai

Chi-Kung Ni

Affiliations

Soon will be listed here.

Abstract

-Linked glycosylation is one of the most essential post-translational modifications of proteins. However, -glycan structural determination remains challenging because of the small differences in structures between isomers. In this study, we constructed a database containing collision-induced dissociation MS mass spectra and chromatograms of high-performance liquid chromatography for the rapid identification of high-mannose and paucimannose -glycan isomers. These -glycans include isomers by breaking of arbitrary numbers of glycosidic bonds at arbitrary positions of canonical ManGlcNAc -glycans. In addition, some GlcManGlcNAc -glycan isomers were included in the database. This database is particularly useful for the identification of the -glycans not in conventional -glycan standards. This study demonstrated the application of the database to structural assignment for high-mannose -glycans extracted from bovine whey proteins, soybean proteins, human mammary epithelial cells, and human breast carcinoma cells. We found many -glycans that are not expected to be generated by conventional biosynthetic pathways of multicellular eukaryotes.

Citing Articles

High Abundance of Unusual High Mannose -Glycans Found in Beans.

Liew C, Luo H, Chen J, Ni C ACS Omega. 2024; 9(46):45822-45827.

PMID: 39583691 PMC: 11579719. DOI: 10.1021/acsomega.4c04114.

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