Simple and Promising Paper-based Electrochemical Platform for Serological Detection of American Tegumentary Leishmaniasis

Overview

Journal Mem Inst Oswaldo Cruz

Specialties Parasitology
Tropical Medicine

Date 2024 Feb 15

PMID 38359306

Authors

Daniela E Barraza

Paula I Nanni

Maria E Bracamonte

Roberto E Chaile

Carla B Goy

Leonardo Acuna

Jorge D Marco

Rossana E Madrid

Affiliations

Soon will be listed here.

Abstract

Background: American tegumentary leishmaniasis (ATL) is an endemic neglected tropical disease (NTD), its conventional treatment is toxic, slow, and invasive. Rapid diagnosis is crucial for the clinical management of suspected patients, so the development and use of low-cost, miniaturised and portable devices could be the key.

Objectives: This work aimed to develop a simple paper-based electrochemical platform for the serological detection of ATL.

Methods: Platform was fabricated in Whatman N°1 paper, contains a hydrophobic zone generated by wax printing, two pencil graphite electrodes, and uses specific crude extracts (CA) antigens for ATL immuno-determination. The platform performance was analysed by measuring the relative impedance change for different antigen-antibody combinations. Then, 10 serum human samples previously diagnosed by the gold standard (five positive ATL cases and five non-ATL cases) were evaluated.

Findings: The platform presented a linear response for the charge transfer resistance (ΔRct) and the interface reactance (ΔXc). Also, optimal working conditions were established (1/60 serum dilution and 180 µg/mL CA concentration). Then, the platform permits to distinguish between ATL and non-ATL (p < 0.05) human serum samples.

Main Conclusions: Our platform could allow the diagnosis, management, and monitoring of leishmaniasis while being an extremely simple and environmentally friendly technology.

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