Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells (SSCs)

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Feb 14

PMID 38351444

Authors

Janmejay Hingu

Guillermo Galdon

Nicholas A Deebel

Hooman Sadri-Ardekani

Affiliations

Soon will be listed here.

Abstract

Preservation of human spermatogonial stem cells (SSCs) may be suitable for young male patients at risk of male infertility due to various causes, such as gonadotoxic treatment or genetic diseases. With optimal cryopreservation, cell viability can be retained to reestablish spermatogenesis in the future through autologous transplantation or in vitro differentiation of SSCs. This protocol outlines techniques to optimize the SSCs isolation and in vitro culture. With particular emphasis on the microscopic characteristics encountered, this protocol outlines a broader approach to processing tissues with varying morphologies among patients.

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