The Role of New Morphological Parameters Provided by the BC 6800 Plus Analyzer in the Early Diagnosis of Sepsis

Overview

Journal Diagnostics (Basel)

Specialty Radiology

Date 2024 Feb 10

PMID 38337856

Authors

Sara Sacchetti

Matteo Vidali

Teresa Esposito

Stefano Zorzi

Alessia Burgener

Lorenzo Ciccarello

Gianmaria Cammarota

Valentina Zanotti

Luca Giacomini

Mattia Bellan

Mario Pirisi

Ramon Simon Lopez

Umberto Dianzani

Rosanna Vaschetto

Roberta Rolla

Affiliations

Soon will be listed here.

Abstract

Background: Late diagnosis of sepsis is associated with adverse consequences and high mortality rate. The aim of this study was to evaluate the diagnostic value of hematologic research parameters, that reflect the cell morphology of blood cells, available on the BC 6800 plus automated analyzer (Mindray) for the early detection of sepsis.

Materials And Methods: A complete blood count (CBC) was performed by Mindray BC 6800 Plus Analyzer in 327 patients (223 with a confirmed diagnosis of sepsis following sepsis-3 criteria, 104 without sepsis), admitted at the Intensive Care Unit of the Novara's Hospital (Italy) and in 56 patients with localized infection.

Results: In univariate logistic regression, age, Hb, RDW, MO#, NMR, NeuX, NeuY, NeuZ, LymX, MonX, MonY, MonZ were associated with sepsis ( < 0.005). In multivariate analysis, only RDW, NeuX, NeuY, NeuZ, MonX and MonZ were found to be independent predictors of sepsis ( < 0.005). Morphological research parameters are confirmed to be predictors of sepsis even when analyzing the group with localized infection.

Conclusions: In addition to already established biomarkers and basic CBC parameters, new morphological cell parameters can be a valuable aid in the early diagnosis of sepsis at no additional cost.

References

Buoro S, Manenti B, Seghezzi M, Dominoni P, Barbui T, Ghirardi A . Innovative haematological parameters for early diagnosis of sepsis in adult patients admitted in intensive care unit. J Clin Pathol. 2017; 71(4):330-335. DOI: 10.1136/jclinpath-2017-204643. View

Hu Z, Lippi G, Montagnana M . Diagnostic and prognostic value of red blood cell distribution width in sepsis: A narrative review. Clin Biochem. 2020; 77:1-6. DOI: 10.1016/j.clinbiochem.2020.01.001. View

Reinhart K, Daniels R, Kissoon N, Machado F, Schachter R, Finfer S . Recognizing Sepsis as a Global Health Priority - A WHO Resolution. N Engl J Med. 2017; 377(5):414-417. DOI: 10.1056/NEJMp1707170. View

Patnaik R, Azim A, Agarwal V . Neutrophil CD64 a Diagnostic and Prognostic Marker of Sepsis in Adult Critically Ill Patients: A Brief Review. Indian J Crit Care Med. 2021; 24(12):1242-1250. PMC: 7775945. DOI: 10.5005/jp-journals-10071-23558. View

Polilli E, Sozio F, Frattari A, Persichitti L, Sensi M, Posata R . Comparison of Monocyte Distribution Width (MDW) and Procalcitonin for early recognition of sepsis. PLoS One. 2020; 15(1):e0227300. PMC: 6953886. DOI: 10.1371/journal.pone.0227300. View

Evans L, Rhodes A, Alhazzani W, Antonelli M, Coopersmith C, French C . Surviving sepsis campaign: international guidelines for management of sepsis and septic shock 2021. Intensive Care Med. 2021; 47(11):1181-1247. PMC: 8486643. DOI: 10.1007/s00134-021-06506-y. View

Urrechaga E, Boveda O, Aguirre U . Role of leucocytes cell population data in the early detection of sepsis. J Clin Pathol. 2017; 71(3):259-266. DOI: 10.1136/jclinpath-2017-204524. View

Arora P, Kumar Gupta P, Lingaiah R, Mukhopadhyay A . Volume, conductivity, and scatter parameters of leukocytes as early markers of sepsis and treatment response. J Lab Physicians. 2019; 11(1):29-33. PMC: 6437820. DOI: 10.4103/JLP.JLP_102_18. View

Montagnana M, Cervellin G, Meschi T, Lippi G . The role of red blood cell distribution width in cardiovascular and thrombotic disorders. Clin Chem Lab Med. 2012; 50(4):635-41. DOI: 10.1515/cclm.2011.831. View

10.

Li R, Tablin F . A Comparative Review of Neutrophil Extracellular Traps in Sepsis. Front Vet Sci. 2018; 5:291. PMC: 6280561. DOI: 10.3389/fvets.2018.00291. View

11.

Ciaccio A, Agnello L, Lo Sasso B, Giglio R, Iacona A, Gambino C . Monocyte Distribution Width (MDW) as a biomarker of sepsis: An evidenced-based laboratory medicine approach. Clin Chim Acta. 2023; 540:117214. DOI: 10.1016/j.cca.2022.117214. View

12.

Lacquaniti A, Ceresa F, Campo S, Barbera G, Caruso D, Palazzo E . Acute Kidney Injury and Sepsis after Cardiac Surgery: The Roles of Tissue Inhibitor Metalloproteinase-2, Insulin-like Growth Factor Binding Protein-7, and Mid-Regional Pro-Adrenomedullin. J Clin Med. 2023; 12(16). PMC: 10455441. DOI: 10.3390/jcm12165193. View

13.

Povoa P, Coelho L, Dal-Pizzol F, Ferrer R, Huttner A, Morris A . How to use biomarkers of infection or sepsis at the bedside: guide to clinicians. Intensive Care Med. 2023; 49(2):142-153. PMC: 9807102. DOI: 10.1007/s00134-022-06956-y. View

14.

van der Poll T, Shankar-Hari M, Wiersinga W . The immunology of sepsis. Immunity. 2021; 54(11):2450-2464. DOI: 10.1016/j.immuni.2021.10.012. View

15.

Molloy E, Bearer C . Paediatric and neonatal sepsis and inflammation. Pediatr Res. 2022; 91(2):267-269. PMC: 8766624. DOI: 10.1038/s41390-021-01918-4. View

16.

Schlapbach L, Kissoon N, Alhawsawi A, Aljuaid M, Daniels R, Gorordo-Delsol L . World Sepsis Day: a global agenda to target a leading cause of morbidity and mortality. Am J Physiol Lung Cell Mol Physiol. 2020; 319(3):L518-L522. DOI: 10.1152/ajplung.00369.2020. View

17.

Shi C, Pamer E . Monocyte recruitment during infection and inflammation. Nat Rev Immunol. 2011; 11(11):762-74. PMC: 3947780. DOI: 10.1038/nri3070. View

18.

Hassan J, Khan S, Zahra R, Razaq A, Zain A, Razaq L . Role of Procalcitonin and C-reactive Protein as Predictors of Sepsis and in Managing Sepsis in Postoperative Patients: A Systematic Review. Cureus. 2022; 14(11):e31067. PMC: 9719405. DOI: 10.7759/cureus.31067. View

19.

Xia X, Wang Y, Xie M, Qiu S, Zhou J . Elevated neutrophil - to - monocyte ratio as a prognostic marker for poor outcomes in neonatal sepsis. Heliyon. 2022; 8(10):e11181. PMC: 9626927. DOI: 10.1016/j.heliyon.2022.e11181. View

20.

Herrero-Cervera A, Soehnlein O, Kenne E . Neutrophils in chronic inflammatory diseases. Cell Mol Immunol. 2022; 19(2):177-191. PMC: 8803838. DOI: 10.1038/s41423-021-00832-3. View