Evaluation of Antimicrobial Susceptibility Tests for Acinetobacter and Pseudomonas Species Using Disks Containing a High Dose of Meropenem

Overview

Journal Sci Rep

Specialty Science

Date 2024 Feb 1

PMID 38302510

Authors

Shoichiro Endo

Tatsuya Tada

Satoshi Oshiro

Tomomi Hishinuma

Mari Tohya

Shin Watanabe

Jun-Ichiro Sekiguchi

Masaki Abe

Koji Nakada

Teruo Kirikae

Affiliations

Soon will be listed here.

Abstract

The emergence and dissemination of carbapenem-resistant species of Acinetobacter and Pseudomonas have become a serious health concern. Routine antimicrobial disk susceptibility tests in clinical laboratories cannot distinguish between isolates that are highly carbapenem-resistant and those that are moderately carbapenem-resistant. The present study describes antimicrobial susceptibility tests using disks containing high doses (1000 μg) of meropenem. The diameters of inhibition zones were significantly negatively correlated with the MICs of Pseudomonas and Acinetobacter species for meropenem (R: 0.93 and 0.91, respectively) and imipenem (R: 0.75 and 0.84, respectively). Double disk synergy tests using clavulanic acid or sodium mercaptoacetate can detect ESBL or MBL producers. Susceptibility tests using disks containing high doses of meropenem can easily detect highly carbapenem-resistant isolates in a quantitative manner. These disks may be useful in bacteriological laboratories because of their technical ease, stability, and relatively low cost.

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