Exploring the Mechanism of Immediate Analgesia Induced by Tuina Intervention on Minor Chronic Constriction Injury in Rats Using LC-MS

Overview

Journal J Pain Res

Publisher Dove Medical Press

Date 2024 Jan 29

PMID 38283563

Authors

Sachula

Zhenjie Yang

Tianyuan Yu

Jinping Chen

Runlong Zhang

Yingqi Zhang

Jiayue Liu

Hanyu Zhang

Jiawei Sun

Affiliations

Soon will be listed here.

Abstract

Purpose: This study aimed to investigate changes in metabolomic expression in the spinal dorsal horn (SDH) and thalamus during a Tuina session, aiming to elucidate the mechanism of immediate analgesia.

Methods: The rats were randomly divided into three groups: the Sham group, the Model group, and the Tuina group. A minor chronic constriction injury (minor CCI) model was established in both the Model group and the Tuina group. The therapeutic effect of Tuina was determined using the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) tests. Differential metabolites of the SDH and thalamus were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatic analysis was performed using CV, PCA, Venn, and KEGG.

Results: The therapeutic effect of MWT and TWL after instant Tuina intervention was significant. The therapeutic effect of Tuina instant was significantly better compared to the Model group. In the Veen analysis, it was found that Tuina instantly regulates 10 differential metabolites in the SDH and 5 differential metabolites in the thalamus. In the KEGG enrichment analysis, we found that differential metabolites were enriched in 43 pathways in the thalamus and 70 pathways in the SDH.

Conclusion: Tuina therapy may have analgesic effects by metabolizing neurotransmitters such as 2-Picolinic Acid, 5-Hydroxy-Tryptophan Glutathione Betaine-aldehyde-chloride Leucine Lysine Methionine Sarcosine Succinic Acid Histidine Acetylcholine and 5-Hydroxyindoleacetic Acid through the cAMP pathway. It also affects pathways of neurodegeneration-multiple diseases, butanoate metabolism, tyrosine metabolism.

Citing Articles

Tuina alleviates the muscle atrophy induced by sciatic nerve injury in rats through regulation of PI3K/Akt signaling.

Zhang Y, Zhang H, Liu J, Sun J, Xu Y, Shi N J Orthop Surg Res. 2024; 19(1):892.

PMID: 39736730 PMC: 11686904. DOI: 10.1186/s13018-024-05270-1.

References

Rondon L, Farges M, Davin N, Sion B, Privat A, Vasson M . L-Arginine supplementation prevents allodynia and hyperalgesia in painful diabetic neuropathic rats by normalizing plasma nitric oxide concentration and increasing plasma agmatine concentration. Eur J Nutr. 2017; 57(7):2353-2363. DOI: 10.1007/s00394-017-1508-x. View

Meng F, Xing H, Su X, Xu W, Song P, Gong L . Analgesic Effect of Tuina on Rat Models with Compression of the Dorsal Root Ganglion Pain. J Vis Exp. 2023; (197). DOI: 10.3791/65535. View

Grace P, Hutchinson M, Manavis J, Somogyi A, Rolan P . A novel animal model of graded neuropathic pain: utility to investigate mechanisms of population heterogeneity. J Neurosci Methods. 2010; 193(1):47-53. DOI: 10.1016/j.jneumeth.2010.08.025. View

Veluchamy A, Hebert H, Meng W, Palmer C, Smith B . Systematic review and meta-analysis of genetic risk factors for neuropathic pain. Pain. 2018; 159(5):825-848. DOI: 10.1097/j.pain.0000000000001164. View

Volchegorskii I, Mester K . [Effects of 3-hydroxypyridine and succinic acid derivatives on the dynamics of dorsalgia and affective disorders after surgical treatment of disc herniation]. Eksp Klin Farmakol. 2010; 73(1):33-9. View

Tanas A, Tozlu O, Gezmis T, Hacimuftuoglu A, Abd El-Aty A, Ceylan O . and Neuroprotective Effects of Sarcosine. Biomed Res Int. 2022; 2022:5467498. PMC: 9587910. DOI: 10.1155/2022/5467498. View

Kimura M, Saito S, Obata H . Dexmedetomidine decreases hyperalgesia in neuropathic pain by increasing acetylcholine in the spinal cord. Neurosci Lett. 2012; 529(1):70-4. DOI: 10.1016/j.neulet.2012.08.008. View

Baron R, Binder A, Wasner G . Neuropathic pain: diagnosis, pathophysiological mechanisms, and treatment. Lancet Neurol. 2010; 9(8):807-19. DOI: 10.1016/S1474-4422(10)70143-5. View

Finnerup N, Kuner R, Jensen T . Neuropathic Pain: From Mechanisms to Treatment. Physiol Rev. 2020; 101(1):259-301. DOI: 10.1152/physrev.00045.2019. View

10.

Huang H, Lyu L, Liu Z, Lyu Z, Huang Y, Wu H . [Intervention effect of pressing and kneading the (GB30) acupoint on NF-κB p65 protein at spinal cord dorsal horn in sciatica rats]. Zhongguo Gu Shang. 2023; 36(6):519-24. DOI: 10.12200/j.issn.1003-0034.2023.06.005. View

11.

Yam M, Loh Y, Tan C, Adam S, Abdul Manan N, Basir R . General Pathways of Pain Sensation and the Major Neurotransmitters Involved in Pain Regulation. Int J Mol Sci. 2018; 19(8). PMC: 6121522. DOI: 10.3390/ijms19082164. View

12.

Bouhassira D . Neuropathic pain: Definition, assessment and epidemiology. Rev Neurol (Paris). 2018; 175(1-2):16-25. DOI: 10.1016/j.neurol.2018.09.016. View

13.

Lv T, Mo Y, Yu T, Shao S, Lu M, Luo Y . Using RNA-Seq to Explore the Repair Mechanism of the Three Methods and Three-Acupoint Technique on DRGs in Sciatic Nerve Injured Rats. Pain Res Manag. 2020; 2020:7531409. PMC: 7298348. DOI: 10.1155/2020/7531409. View

14.

Jensen T, Baron R, Haanpaa M, Kalso E, Loeser J, Rice A . A new definition of neuropathic pain. Pain. 2011; 152(10):2204-2205. DOI: 10.1016/j.pain.2011.06.017. View

15.

Hongye H, BingQian W, Shuijin C, Jiayu F, Xiaohua W, Lechun C . Chinese Tuina remodels the synaptic structure in neuropathic pain rats by downregulating the expression of N-methyl D-aspartate receptor subtype 2B and postsynaptic density protein-95 in the spinal cord dorsal horn. J Tradit Chin Med. 2023; 43(4):715-724. PMC: 10626369. DOI: 10.19852/j.cnki.jtcm.20221214.002. View

16.

Bannister K, Sachau J, Baron R, Dickenson A . Neuropathic Pain: Mechanism-Based Therapeutics. Annu Rev Pharmacol Toxicol. 2020; 60:257-274. DOI: 10.1146/annurev-pharmtox-010818-021524. View

17.

Wood J, Kurylo E, Geddes J . Methionine-induced changes in glutamate, aspartate, glutamine, and gamma-aminobutyrate levels in brain tissue. J Neurochem. 1985; 45(3):777-83. DOI: 10.1111/j.1471-4159.1985.tb04060.x. View

18.

Li J, Tian M, Hua T, Wang H, Yang M, Li W . Combination of autophagy and NFE2L2/NRF2 activation as a treatment approach for neuropathic pain. Autophagy. 2021; 17(12):4062-4082. PMC: 8726676. DOI: 10.1080/15548627.2021.1900498. View

19.

Bai J, Wang D, Liu Z, Zhang J, Liu L, Han Y . [Simultaneous determination of amino acid and monoamine neurotransmitters in serum by high performance liquid chromatography coupled with precolumn derivatization]. Se Pu. 2021; 38(8):923-928. DOI: 10.3724/SP.J.1123.2019.12029. View

20.

Walikonis R, Poduslo J . Activity of cyclic AMP phosphodiesterases and adenylyl cyclase in peripheral nerve after crush and permanent transection injuries. J Biol Chem. 1998; 273(15):9070-7. DOI: 10.1074/jbc.273.15.9070. View