Comprehensive Analysis of MiRNA Profiling in Schistosoma Mekongi Across Life Cycle Stages

Overview

Journal Sci Rep

Specialty Science

Date 2024 Jan 28

PMID 38281987

Authors

Pavaret Sivapornnukul

Ariya Khamwut

Prangwalai Chanchaem

Phiraphol Chusongsang

Yupa Chusongsang

Paporn Poodeepiyasawat

Yanin Limpanont

Onrapak Reamtong

Sunchai Payungporn

Affiliations

Soon will be listed here.

Abstract

Schistosoma mekongi, a significant schistosome parasite, has various life stages, including egg, cercaria, female, and male, that play crucial roles in the complex life cycle. This study aimed to explore the microRNA (miRNA) profiles across these developmental stages to understand their potential functions and evolutionary significance, which have not been studied. Pre-processed sequencing reads of small RNA (sRNA) were obtained, and annotations were performed against the S. japonicum reference miRNA database. Results indicated marked variations in miRNA profiles across different life stages, with notable similarities observed between female and male S. mekongi. Principal Coordinate Analysis (PCoA) and unsupervised clustering revealed distinct miRNA signatures for each stage. Gene ontology (GO) analysis unveiled the potential roles of these miRNAs in various biological processes. The differential expression of specific miRNAs was prominent across stages, suggesting their involvement in crucial developmental processes. Furthermore, orthologous miRNA analysis against various worm species revealed distinct presence-absence patterns, providing insights into the evolutionary relationships of these miRNAs. In conclusion, this comprehensive investigation into the miRNA profiles of S. mekongi offers valuable insights into the functional and evolutionary aspects of miRNAs in schistosome biology.

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