Suitability of Ex Vivo-Expanded Microtic Perichondrocytes for Auricular Reconstruction

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2024 Jan 22

PMID 38247833

Authors

Yvonne Jakob

Johann Kern

David Gvaramia

Philipp Fisch

Ralph Magritz

Sven Reutter

Nicole Rotter

Affiliations

Soon will be listed here.

Abstract

Tissue engineering (TE) techniques offer solutions for tissue regeneration but require large quantities of cells. For microtia patients, TE methods represent a unique opportunity for therapies with low donor-site morbidity and reliance on the surgeon's individual expertise. Microtia-derived chondrocytes and perichondrocytes are considered a valuable cell source for autologous reconstruction of the pinna. The aim of this study was to investigate the suitability of perichondrocytes from microtia patients for autologous reconstruction in comparison to healthy perichondrocytes and microtia chondrocytes. Perichondrocytes were isolated via two different methods: explant culture and enzymatic digestion. The isolated cells were analyzed in vitro for their chondrogenic cell properties. We examined migration activity, colony-forming ability, expression of mesenchymal stem cell markers, and gene expression profile. We found that microtic perichondrocytes exhibit similar chondrogenic properties compared to chondrocytes in vitro. We investigated the behavior in three-dimensional cell cultures (spheroids and scaffold-based 3D cell cultures) and assessed the expression of cartilage-specific proteins via immunohistochemistry, e.g., collagen II, which was detected in all samples. Our results show that perichondrocytes from microtia patients are comparable to healthy perichondrocytes and chondrocytes in terms of chondrogenic cell properties and could therefore be a promising cell source for auricular reconstruction.

Citing Articles

[Research progress of three-dimensional bioprinting technology in auricle repair and reconstruction].

Chen X, Hu H, Li Y, Yue W, Zhang X, Shen D Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2024; 38(6):763-768.

PMID: 38918200 PMC: 11190673. DOI: 10.7507/1002-1892.202403001.

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