Dual Phosphorylation of DGK5-mediated PA Burst Regulates ROS in Plant Immunity

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 2024 Jan 20

PMID 38244548

Authors

Liang Kong

Xiyu Ma

Chao Zhang

Sung-Il Kim

Bo Li

Yingpeng Xie

In-Cheol Yeo

Hem Thapa

Sixue Chen

Timothy P Devarenne

Teun Munnik

Ping He

Libo Shan

Affiliations

Soon will be listed here.

Abstract

Phosphatidic acid (PA) and reactive oxygen species (ROS) are crucial cellular messengers mediating diverse signaling processes in metazoans and plants. How PA homeostasis is tightly regulated and intertwined with ROS signaling upon immune elicitation remains elusive. We report here that Arabidopsis diacylglycerol kinase 5 (DGK5) regulates plant pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). The pattern recognition receptor (PRR)-associated kinase BIK1 phosphorylates DGK5 at Ser-506, leading to a rapid PA burst and activation of plant immunity, whereas PRR-activated intracellular MPK4 phosphorylates DGK5 at Thr-446, which subsequently suppresses DGK5 activity and PA production, resulting in attenuated plant immunity. PA binds and stabilizes the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD), regulating ROS production in plant PTI and ETI, and their potentiation. Our data indicate that distinct phosphorylation of DGK5 by PRR-activated BIK1 and MPK4 balances the homeostasis of cellular PA burst that regulates ROS generation in coordinating two branches of plant immunity.

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