Opsin-Free Activation of Bmp Receptors by a Femtosecond Laser

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2024 Jan 19

PMID 38239021

Authors

Manjun Xu

Haipeng Wang

Xiaoying Tian

Bingyi Li

Shaoyang Wang

Xiaohui Zhao

Hao He

Affiliations

Soon will be listed here.

Abstract

Bone morphogenetic protein (BMP) signaling plays a vital role in differentiation, organogenesis, and various cell processes. As a member of TGF-β superfamily, the BMP initiation usually accompanies crosstalk with other signaling pathways and simultaneously activates some of them. It is quite challenging to solely initiate an individual pathway. In this study, an opsin-free optical method to specifically activate BMP receptors (BMPR) and subsequent pSmad1/5/8 cascades by a single-time scan of a tightly-focused femtosecond laser in the near infrared range is reported. Via transient two-photon excitation to intrinsic local flavins near the cell membrane, the photoactivation drives conformational changes of preformed BMPR complexes to enable their bonding and phosphorylation of the GS domain in BMPR-I by BMPR-II. The pSmad1/5/8 signaling is initiated by this method, while p38 and pSmad2 are rarely perturbed. Based on a microscopic system, primary adipose-derived stem cells in an area of 420 × 420 µm are photoactivated by a single-time laser scanning for 1.5 s and exhibit pSmad1/5/8 upregulation and osteoblastic differentiation after 21 days. Hence, an opsin-free, specific, and noninvasive optical method to initiate BMP signaling, easily accomplished by a two-photon microscope system is reported.

Citing Articles

Opsin-Free Activation of Bmp Receptors by a Femtosecond Laser.

Xu M, Wang H, Tian X, Li B, Wang S, Zhao X Adv Sci (Weinh). 2024; 11(10):e2308072.

PMID: 38239021 PMC: 10933676. DOI: 10.1002/advs.202308072.

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