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Application of Immobilized Enzymes in Juice Clarification

Overview
Journal Foods
Specialty Biotechnology
Date 2024 Jan 17
PMID 38231709
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Abstract

Immobilized enzymes are currently being rapidly developed and are widely used in juice clarification. Immobilized enzymes have many advantages, and they show great advantages in juice clarification. The commonly used methods for immobilizing enzymes include adsorption, entrapment, covalent bonding, and cross-linking. Different immobilization methods are adopted for different enzymes to accommodate their different characteristics. This article systematically reviews the methods of enzyme immobilization and the use of immobilized supports in juice clarification. In addition, the mechanisms and effects of clarification with immobilized pectinase, immobilized laccase, and immobilized xylanase in fruit juice are elaborated upon. Furthermore, suggestions and prospects are provided for future studies in this area.

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References
1.
Shallom D, Shoham Y . Microbial hemicellulases. Curr Opin Microbiol. 2003; 6(3):219-28. DOI: 10.1016/s1369-5274(03)00056-0. View

2.
Del Arco J, Alcantara A, Fernandez-Lafuente R, Fernandez-Lucas J . Magnetic micro-macro biocatalysts applied to industrial bioprocesses. Bioresour Technol. 2020; 322:124547. DOI: 10.1016/j.biortech.2020.124547. View

3.
Upadhyay P, Shrivastava R, Agrawal P . Bioprospecting and biotechnological applications of fungal laccase. 3 Biotech. 2017; 6(1):15. PMC: 4703590. DOI: 10.1007/s13205-015-0316-3. View

4.
Kharazmi S, Taheri-Kafrani A, Soozanipour A, Nasrollahzadeh M, Varma R . Xylanase immobilization onto trichlorotriazine-functionalized polyethylene glycol grafted magnetic nanoparticles: A thermostable and robust nanobiocatalyst for fruit juice clarification. Int J Biol Macromol. 2020; 163:402-413. DOI: 10.1016/j.ijbiomac.2020.06.273. View

5.
Li X, Jiang Z, Li L, Yang S, Feng W, Fan J . Characterization of a cellulase-free, neutral xylanase from Thermomyces lanuginosus CBS 288.54 and its biobleaching effect on wheat straw pulp. Bioresour Technol. 2005; 96(12):1370-9. DOI: 10.1016/j.biortech.2004.11.006. View