Immunomodulatory Activity and Its Mechanisms of Two Polysaccharides from

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2024 Jan 11

PMID 38202633

Authors

Wuxia Zhang

Jiaqi He

Danping Zheng

Panpan Zhao

Yingdong Wang

Jinzhong Zhao

Peng Li

Affiliations

Soon will be listed here.

Abstract

Polyporaceae is an important fungal family that has been a source of natural products with a range of pharmaceutical activities in China. In our previous study, two polysaccharides, PCWPW and PCWPS, with significant antioxidant and antidepressant activity were obtained from . In this study, we evaluated their potential molecular mechanisms in the immunomodulation of macrophages. PCWPW and PCWPS were characterized by GC-MS analysis to contain 1,3-linked Glcp. ELISA assays results demonstrated that the secretion of TNF-α was significantly enhanced by PCWPW/PCWPS. RNA-seq data demonstrated that PCWPS treatment modulated the expression of immune-related genes in macrophages, which was further confirmed by RT-qPCR assays. The activation of TNF-α secretion was found to be mannose receptor (MR) dependent and suppressed by MR inhibitor pretreatment. Moreover, the amount of TNF-α cytokine secretion in PCWPW/PCWPS-induced RAW264.7 cells was decreased when pretreated with NF-κB or MAPK signaling pathway inhibitors. Collectively, our results suggested that PCWPW and PCWPS possessed immunomodulatory activity that regulates TNF-α expression through the NF-κB/MAPK signaling pathway by binding to mannose receptors. Therefore, PCWPW and PCWPS isolated from have potential as drug candidates for immune-related disease treatment.

Citing Articles

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References

Zhang W, Chen L, Li P, Zhao J, Duan J . Antidepressant and immunosuppressive activities of two polysaccharides from Poria cocos (Schw.) Wolf. Int J Biol Macromol. 2018; 120(Pt B):1696-1704. DOI: 10.1016/j.ijbiomac.2018.09.171. View

Li K, Cao Y, Jiao S, Du G, Du Y, Qin X . Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix. Front Pharmacol. 2021; 11:582091. PMC: 7774520. DOI: 10.3389/fphar.2020.582091. View

Du J, Li J, Zhu J, Huang C, Bi S, Song L . Structural characterization and immunomodulatory activity of a novel polysaccharide from Ficus carica. Food Funct. 2018; 9(7):3930-3943. DOI: 10.1039/c8fo00603b. View

Wu Y, Zhou H, Wei K, Zhang T, Che Y, Nguyn A . Structure of a new glycyrrhiza polysaccharide and its immunomodulatory activity. Front Immunol. 2022; 13:1007186. PMC: 9551306. DOI: 10.3389/fimmu.2022.1007186. View

Wu Y, Li D, Wang H, Wan X . Protective Effect of Poria Cocos Polysaccharides on Fecal Peritonitis-Induced Sepsis in Mice Through Inhibition of Oxidative Stress, Inflammation, Apoptosis, and Reduction of Treg Cells. Front Microbiol. 2022; 13:887949. PMC: 9184799. DOI: 10.3389/fmicb.2022.887949. View

Zeng P, Li J, Chen Y, Zhang L . The structures and biological functions of polysaccharides from traditional Chinese herbs. Prog Mol Biol Transl Sci. 2019; 163:423-444. PMC: 7102684. DOI: 10.1016/bs.pmbts.2019.03.003. View

Talapphet N, Palanisamy S, Li C, Ma N, Prabhu N, You S . Polysaccharide extracted from Taraxacum platycarpum root exerts immunomodulatory activity via MAPK and NF-κB pathways in RAW264.7 cells. J Ethnopharmacol. 2021; 281:114519. DOI: 10.1016/j.jep.2021.114519. View

Liu Y, Li Q, Li L, Zhou X . Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways. J Ethnopharmacol. 2021; 275:114130. DOI: 10.1016/j.jep.2021.114130. View

Zhang W, Wang S, Kang C, Lv C, Zhou L, Huang L . Pharmacodynamic material basis of traditional Chinese medicine based on biomacromolecules: a review. Plant Methods. 2020; 16:26. PMC: 7049221. DOI: 10.1186/s13007-020-00571-y. View

10.

Li X, Zhang Q, Zhu Y, Li Y, Mei S, Luo H . Structural characterization of a mannoglucan polysaccharide from Dendrobium huoshanense and evaluation of its osteogenesis promotion activities. Int J Biol Macromol. 2022; 211:441-449. DOI: 10.1016/j.ijbiomac.2022.05.036. View

11.

Jiang Y, Wang L, Chen W, Duan Y, Sun M, Huang J . polysaccharide prevents alcohol-induced hepatic injury and inflammation by repressing oxidative stress and gut leakiness. Front Nutr. 2022; 9:963598. PMC: 9428680. DOI: 10.3389/fnut.2022.963598. View

12.

Guo Y, Li S, Huang P, Zhang H, Yu C . Development of a prognostic model based on an immunogenomic landscape analysis of medulloblastoma. Biosci Rep. 2020; 41(1). PMC: 7791544. DOI: 10.1042/BSR20202907. View

13.

Tan M, Chang S, Liu J, Li H, Xu P, Wang P . Physicochemical Properties, Antioxidant and Antidiabetic Activities of Polysaccharides from Quinoa ( Willd.) Seeds. Molecules. 2020; 25(17). PMC: 7503530. DOI: 10.3390/molecules25173840. View

14.

Jeong M, Kim Y, Kim A, Jung J, Son M . The herbal drug, Bu-Zhong-Yi-Qi-Tang, for the treatment of atopic dermatitis: Protocol for a systematic review. Medicine (Baltimore). 2019; 98(1):e13938. PMC: 6344144. DOI: 10.1097/MD.0000000000013938. View

15.

Li Y, Jin T, Liu N, Wang J, Qin Z, Yin S . A short peptide exerts neuroprotective effects on cerebral ischemia-reperfusion injury by reducing inflammation via the miR-6328/IKKβ/NF-κB axis. J Neuroinflammation. 2023; 20(1):53. PMC: 9972639. DOI: 10.1186/s12974-023-02739-4. View

16.

Qin L, Chen S, Xie L, Yu Q, Chen Y, Shen M . Mechanisms of RAW264.7 macrophages immunomodulation mediated by polysaccharide from mung bean skin based on RNA-seq analysis. Food Res Int. 2022; 154:111017. DOI: 10.1016/j.foodres.2022.111017. View

17.

Duan Y, Huang J, Sun M, Jiang Y, Wang S, Wang L . Poria cocos polysaccharide improves intestinal barrier function and maintains intestinal homeostasis in mice. Int J Biol Macromol. 2023; 249:125953. DOI: 10.1016/j.ijbiomac.2023.125953. View

18.

Duan G, Yu X . Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia. Int J Biol Macromol. 2019; 137:1112-1120. DOI: 10.1016/j.ijbiomac.2019.06.177. View

19.

Shu H, Lin Y, Zhang Z, Qiu L, Ding W, Gao Q . The transcriptomic profile of Spodoptera frugiperda differs in response to a novel insecticide, cyproflanilide, compared to chlorantraniliprole and avermectin. BMC Genomics. 2023; 24(1):3. PMC: 9811769. DOI: 10.1186/s12864-022-09095-2. View

20.

Li L, Zuo Z, Wang Y . The Traditional Usages, Chemical Components and Pharmacological Activities of : A Review. Am J Chin Med. 2022; 50(2):389-440. DOI: 10.1142/S0192415X22500161. View