MosGILT Controls Innate Immunity and Germ Cell Development in Anopheles Gambiae

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2024 Jan 8

PMID 38191283

Authors

Gunjan Arora

Xiaotian Tang

Yingjun Cui

Jing Yang

Yu-Min Chuang

Jayadev Joshi

Andaleeb Sajid

Yuemei Dong

Peter Cresswell

George Dimopoulos

Erol Fikrig

Affiliations

Soon will be listed here.

Abstract

Gene-edited mosquitoes lacking a gamma-interferon-inducible lysosomal thiol reductase-like protein, namely (mosGILT) have lower Plasmodium infection, which is linked to impaired ovarian development and immune activation. The transcriptome of mosGILT Anopheles gambiae was therefore compared to wild type (WT) mosquitoes by RNA-sequencing to delineate mosGILT-dependent pathways. Compared to WT mosquitoes, mosGILT A. gambiae demonstrated altered expression of genes related to oogenesis, 20-hydroxyecdysone synthesis, as well as immune-related genes. Serendipitously, the zero population growth gene, zpg, an essential regulator of germ cell development was found to be one of the most downregulated genes in mosGILT mosquitoes. These results provide a crucial missing link between two previous studies on the role of zpg and mosGILT in ovarian development. This study further demonstrates that mosGILT has the potential to serve as a target for the biological control of mosquito vectors and to influence the Plasmodium life cycle within the vector.

Citing Articles

mosGILT antibodies interfere with Plasmodium sporogony in Anopheles gambiae.

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