Grass Carp Reovirus VP56 and VP35 Induce Formation of Viral Inclusion Bodies for Replication

Overview

Journal iScience

Publisher Cell Press

Date 2024 Jan 8

PMID 38188516

Authors

Chu Zhang

Hui Wu

Hao Feng

Yong-An Zhang

Jiagang Tu

Affiliations

Soon will be listed here.

Abstract

Viral inclusion bodies (VIBs) are subcellular structures required for efficient viral replication. How type II grass carp reovirus (GCRV-II), the mainly prevalent strain, forms VIBs is unknown. In this study, we found that GCRV-II infection induced punctate VIBs in grass carp ovary (GCO) cells and that non-structural protein 38 (NS38) functioned as a participant in VIB formation. Furthermore, VP56 and VP35 induced VIBs and recruited other viral proteins via the N-terminal of VP56 and the middle domain of VP35. Additionally, we found that the newly synthesized viral RNAs co-localized with VP56 and VP35 in VIBs during infection. Taken together, VP56 and VP35 induce VIB formation and recruit other viral proteins and viral RNAs to the VIBs for viral replication, which helps identify new targets for developing anti-GCRV-II drugs to disrupt viral replication.

Citing Articles

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