Mycotoxins from , the Specific Plant Pathogen of

Ginseng black spot, caused by , is one of the most common diseases of , which usually causes serious yield loss of ginseng plants. However, the pathogenic mechanism of has not been clarified clearly. Mycotoxins produced by phytopathogens play an important role in the process of infection. Previous study reported that dibutyl phthalate (DBP) identified from the metabolites of is a potent mycotoxin against . However, more evidence suggests that DBP is one of the constituents of plasticisers. To identify mycotoxins from and evaluate their phytotoxicity on the leaves of , different chromatographic, spectral and bioassay-guided methods were used together in this report. As a result, tyrosol (), 3-hydroxy-3-(4-methoxyphenyl) propanoic acid (), and 3-benzylpiperazine-2,5-dione () were isolated and characterised from the extract of , in which compounds and showed phytotoxic activity on ginseng leaves. Furthermore, DBP was confirmed to come from the residue of ethyl acetate through UPLC-MS/MS analysis, and displayed no phytotoxicity on ginseng leaves based on biological experiments. The results in this report first revealed that tyrosol (), and 3-hydroxy-3-(4-methoxyphenyl) propanoic acid () not DBP were the potent mycotoxins of .