ApWD40a, a Member of the WD40-Repeat Protein Family, Is Crucial for Fungal Development, Toxin Synthesis, and Pathogenicity in the Ginseng Alternaria Leaf Blight Fungus

Overview

Journal J Fungi (Basel)

Date 2025 Jan 24

PMID 39852478

Authors

Jinling Lan

Shengjie Mei

Yingxue Du

Meili Chi

Jiayi Yang

Shuliu Guo

Mingliang Chu

Ronglin He

Jie Gao

Affiliations

Soon will be listed here.

Abstract

, the primary pathogen that causes ginseng Alternaria leaf blight disease, can lead to a 20-30% reduction in ginseng yield. WD40 repeat-containing proteins are evolutionarily conserved proteins with diverse functions between different organisms. In this study, we characterized the roles of a WD40 repeat-containing protein in . The deletion of impaired the mycelial growth, reduced the sporulation, and significantly decreased the efficiency in utilizing various carbon sources. The Δ mutant showed increased sensitivity to osmotic stress and metal ion stress induced by sorbitol, NaCl, and KCl, but decreased the sensitivity to a cell wall stress factor (SDS) and oxidative stress factors (paraquat and HO). Pathogenicity assays performed on detached ginseng leaves and roots revealed that the disruption of significantly decreased the fungal virulence through attenuating melanin and mycotoxin production by . A comparative transcriptome analysis revealed that was involved in many metabolic and biosynthetic processes, including amino acid metabolism, carbon metabolism, sulfate metabolic pathways, and secondary metabolite pathways. In particular, a significantly upregulated gene that encoded a sulfate permease 2 protein in Δ, named , was deleted in the wild-type strain of . The deletion of resulted in reduced biomass under sulfate-free conditions, demonstrating that the sulfate transport was impaired. Taken together, our findings highlight that played crucial roles in different biological processes and the pathogenicity of through modulating the expressions of genes involved in various primary and secondary metabolic processes.

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