An Integrated Analysis of Bulk and Single-cell Sequencing Data Reveals That EMP1/COL3A1 Fibroblasts Contribute to the Bone Metastasis Process in Breast, Prostate, and Renal Cancers

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2024 Jan 8

PMID 38187400

Authors

Haoyuan Du

Hua Wang

Yuwei Luo

Yang Jiao

Jiajun Wu

Shaowei Dong

Dong Du

Affiliations

Soon will be listed here.

Abstract

Introduction: Bone metastasis (BoM) occurs when cancer cells spread from their primary sites to a bone. Currently, the mechanism underlying this metastasis process remains unclear.

Methods: In this project, through an integrated analysis of bulk-sequencing and single-cell RNA transcriptomic data, we explored the BoM-related features in tumor microenvironments of different tumors.

Results: We first identified 34 up-regulated genes during the BoM process in breast cancer, and further explored their expression status among different components in the tumor microenvironment (TME) of BoM samples. Enriched EMP1+ fibroblasts were found in BoM samples, and a COL3A1-ADGRG1 communication between these fibroblasts and cancer cells was identified which might facilitate the BoM process. Moreover, a significant correlation between EMP1 and COL3A1 was identified in these fibroblasts, confirming the potential connection of these genes during the BoM process. Furthermore, the existence of these EMP1+/COL3A1+ fibroblasts was also verified in prostate cancer and renal cancer BoM samples, suggesting the importance of these fibroblasts from a pan-cancer perspective.

Discussion: This study is the first attempt to investigate the relationship between fibroblasts and BoM process across multi-tumor TMEs. Our findings contribute another perspective in the exploration of BoM mechanism while providing some potential targets for future treatments of tumor metastasis.

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PMID: 39353900 PMC: 11445230. DOI: 10.1038/s41419-024-07095-6.

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