Protocol to Study the Inheritance and Propagation of Non-genetically Encoded States Using Barcode Decay Lineage Tracing

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2024 Jan 5

PMID 38180835

Authors

Yelyzaveta Shlyakhtina

Bianca Bloechl

Katherine L Moran

Maximiliano M Portal

Affiliations

Soon will be listed here.

Abstract

Here, we present a protocol to perform barcode decay lineage tracing followed by single-cell transcriptome analysis (BdLT-Seq). We describe steps for BdLT-Seq experimental design, building barcoded episome reporters, performing episome transfection, and barcode retrieval. We then describe procedures for sequencing library construction while providing options for sample multiplexing and data analysis. This BdLT-Seq technique enables the assessment of clonal evolution in a directional manner while preserving isogeneity, thus allowing the comparison of non-genetic molecular features between isogenic cell lineages. For complete details on the use and execution of this protocol, please refer to Shlyakhtina et al. (2023)..

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