Protocol for Transplanting Pancreatic Islets into the Parametrial Fat Pad of Female Mice

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2024 Jan 5

PMID 38180833

Authors

Mebrahtu G Tedla

Nathaniel Wright

Esma S Yolcu

Yadong Wang

Haval Shirwan

Affiliations

Soon will be listed here.

Abstract

Although the male epididymal fat pad is an effective site for islet transplantation, females lack this tissue. Here, we present a protocol to assess the parametrial fat pad (PFP) adjacent to the uterine horn in females as an alternative site for islet transplantation. We describe steps for islet isolation from the pancreas, counting, transplantation into PFP, and monitoring for engraftment. Transplantation into PFP is minimally invasive, time efficient, and supports long-term engraftment of syngeneic islets and rejection of allogeneic islets. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022)..

Citing Articles

Protocol for isolation and transplantation of rat pancreatic islets in the kidney capsule to treat diabetes.

Li D, Kong W, Hong Y, Zhang W, Sun B, Wang X STAR Protoc. 2024; 6(1):103486.

PMID: 39673701 PMC: 11699416. DOI: 10.1016/j.xpro.2024.103486.

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