Benign and Malignant Prostate Neoplasms Show Different Spatial Organization of Collagen

Overview

Journal Croat Med J

Specialty General Medicine

Date 2024 Jan 3

PMID 38168522

Authors

Taras Zadvornyi

Nataliia Lukianova

Oleksandr Mushii

Anna Pavlova

Olena Voronina

Vasyl Chekhun

Affiliations

Soon will be listed here.

Abstract

Aim: To compare the indicators of the spatial organization of collagen and its regulating factors between benign and malignant prostate neoplasms.

Methods: The study involved tumor tissue samples from 40 patients with stage II-III prostate cancer (PCa) and 20 patients with benign prostatic hyperplasia (BPH). The localization of collagen was determined with a Masson trichrome stain. To establish quantitative indicators of the spatial organization of collagen, morphometric studies were carried out with the CurveAlign and ImageJ programs.

Results: PCa tissue had two times lower collagen density (P<0.0001) and 1.3 times lower levels of collagen alignment (P=0.018) compared with BPH tissue. In PCa tissue, collagen fibers were shorter (by 24.2%; P<0.001) and thicker (by 15.5%; P<0.001). PCa tissue samples showed significantly higher levels of metalloproteinase (MMP)-2 (by 2.4 times; P=0.001), MMP-8 (by 2.3 times; P=0.007), and MMP-13 (by 1.9 times; P=0.004).

Conclusions: Collagen matrix spatial organization features, as well as its regulatory factors, could be potential biomarkers of malignant prostate neoplasms.

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