Development of Organoids to Study Infectious Host Interactions

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Jan 2

PMID 38165622

Authors

Aparajita Lahree

Leona Gilbert

Affiliations

Soon will be listed here.

Abstract

Emerging organoid research is paving way for studies in infectious diseases. Described here is a technique for the generation of stem-cell derived organoids for human small intestine and lung together with methods to infect such organoids with a mock pathogen (Cryptosporidium parvum). Such systems are amenable to imaging and processing for molecular biological analyses. It is the intent of this chapter to provide a simple, routine organoid procedure so that in vitro studies with Borrelia such as cell invasion and dissemination can be conducted.

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