Brain Tissue Haemoglobin Expression in Saline-perfused Vs Non-perfused Rodents

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Jan 1

PMID 38163098

Authors

Marion Walser

Lars Karlsson

Reza Motalleb

Jorgen Isgaard

H Georg Kuhn

N David Aberg

Affiliations

Soon will be listed here.

Abstract

Haemoglobin beta () and delta-aminolevulinate synthase 2 () messenger RNA (mRNA) is mainly found in immature red blood cells, reticulocytes, and not in mature erythrocytes. However, these are also expressed in other tissues such as brain cells, mostly neurons. Therefore, exact quantification of neural tissue homogenates may be confounded by remaining blood in the brain vasculature that may give falsely high values of expression. To investigate and compare the contribution of local expression, we investigated mRNA expression locally in the hippocampus and prefrontal cortex, in post-sacrifice saline-perfused and non-perfused mice and rats. Although there was a higher level of transcripts in the non-perfused animals, there was a significant mRNA expression in perfused brains that could at most partially be explained by remaining blood. Finally, we suggest that saline-perfusion should be recommended for quantification of brain transcripts in homogenates.

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