Progresses in Cell-Free In Vitro Evolution

Overview

Journal Adv Biochem Eng Biotechnol

Specialties Biochemistry
Biotechnology

Date 2023 Jun 12

PMID 37306699

Authors

Kaito Seo

Katsumi Hagino

Norikazu Ichihashi

Affiliations

Soon will be listed here.

Abstract

Biopolymers, such as proteins and RNA, are integral components of living organisms and have evolved through a process of repeated mutation and selection. The technique of "cell-free in vitro evolution" is a powerful experimental approach for developing biopolymers with desired functions and structural properties. Since Spiegelman's pioneering work over 50 years ago, biopolymers with a wide range of functions have been developed using in vitro evolution in cell-free systems. The use of cell-free systems offers several advantages, including the ability to synthesize a wider range of proteins without the limitations imposed by cytotoxicity, and the capacity for higher throughput and larger library sizes than cell-based evolutionary experiments. In this chapter, we provide a comprehensive overview of the progress made in the field of cell-free in vitro evolution by categorizing evolution into directed and undirected. The biopolymers produced by these methods are valuable assets in medicine and industry, and as a means of exploring the potential of biopolymers.

Citing Articles

Cell-free expression of RuBisCO for ATP production in the synthetic cells.

Sugii S, Hagino K, Mizuuchi R, Ichihashi N Synth Biol (Oxf). 2023; 8(1):ysad016.

PMID: 38149045 PMC: 10750972. DOI: 10.1093/synbio/ysad016.

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